SME-4-producing Serratia marcescens from Argentina belonging to clade 2 of the S. marcescens phylogeny

Laura Dabos (1, 2) , Rafael Patino-Navarrete (2) , Marcela Nastro (3) , Angela Famiglietti (3) , Philippe Glaser (2) , Carlos Rodriguez (4, 3) , Thierry Naas (5, 2, 1, 6)

1 Structure, Dynamique, Fonction Et Expression Des Beta-Lactamases À Large Spectre
2 EERA - Ecologie et Evolution de la Résistance aux Antibiotiques / Ecology and Evolution of Antibiotics Resistance
3 Laboratorio de Bacteriología Clínica [Buenos Aires]
4 UBA - Universidad de Buenos Aires [Buenos Aires]
5 Hôpital Bicêtre [AP-HP, Le Kremlin-Bicêtre]
6 CNRARA/Service de Microbiologie - Centre National de Référence Associé de la Résistance aux Antibiotiques [Hôpital Bicêtre AP-HP]

Background: SME carbapenemases are increasingly reported, especially from North and South America. Here, we describe an SME-4-producing Serratia marcescens (SME-Sm) clinical isolate from Argentina and compare its genome with other SME-Sm and Sm isolates recovered from public databases. Methods: Sm isolates were characterized by WGS using Illumina technology, susceptibility testing and MIC determination. Carbapenemase activity was revealed by biochemical tests based on imipenem hydrolysis. A whole-genome phylogeny was estimated for all the Sm isolates retrieved from public databases with kSNP3 and a whole-genome phylogenetic analysis based on non-recombinant core SNPs was inferred for Sm complete genomes and for those encoding any bla SME variants.

Domaines: Biologie moléculaire

Laura Dabos, Rafael Patino-Navarrete, Marcela Nastro, Angela Famiglietti, Philippe Glaser, et al.. SME-4-producing Serratia marcescens from Argentina belonging to clade 2 of the S. marcescens phylogeny. Journal of Antimicrobial Chemotherapy, 2019, 74 (7), pp.1836-1841. ⟨10.1093/jac/dkz115⟩. ⟨pasteur-02565488⟩