Macromolecular crowding links ribosomal protein gene dosage to growth rate in Vibrio cholerae

Alfonso Soler-Bistué (1, 2) , Sebastián Aguilar-Pierlé (1) , Marc Garcia-Garcerà (3) , Marie-Eve Val (1) , Odile Sismeiro (4) , Hugo Varet (5, 4) , Rodrigo Sieira (6) , Evelyne Krin (1) , Ole Skovgaard (7) , Diego J Comerci (2) , Eduardo P C Rocha (3) , Didier Mazel (1)

1 PGB - Plasticité du Génome Bactérien - Bacterial Genome Plasticity
2 UNSAM - Universidad Nacional de San Martin
3 Génomique évolutive des Microbes / Microbial Evolutionary Genomics
4 PF2 - Transcriptome et Epigénome
5 Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB
6 Fundación Instituto Leloir [Buenos Aires]
7 Roskilde University

Background: In fast-growing bacteria, the genomic location of ribosomal protein (RP) genes is biased towards the replication origin (oriC). This trait allows optimizing their expression during exponential phase since oriC neighboring regions are in higher dose due to multifork replication. Relocation of s10-spc-α locus (S10), which codes for most of the RP, to ectopic genomic positions shows that its relative distance to the oriC correlates to a reduction on its dosage, its expression, and bacterial growth rate. However, a mechanism linking S10 dosage to cell physiology has still not been determined.

Keywords: Bacterial chromosome; Bacterial physiology; Growth rate; Macromolecular crowding; Ribosomal proteins; Synthetic biology; Vibrio cholerae

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Molecular biology

Alfonso Soler-Bistué, Sebastián Aguilar-Pierlé, Marc Garcia-Garcerà, Marie-Eve Val, Odile Sismeiro, et al.. Macromolecular crowding links ribosomal protein gene dosage to growth rate in Vibrio cholerae. BMC Biology, 2020, 18 (1), pp.43. ⟨10.1186/s12915-020-00777-5⟩. ⟨pasteur-02562489⟩