Expansion of the SOS regulon of Vibrio cholerae through extensive transcriptome analysis and experimental validation

Evelyne Krin (1) , Sebastian Aguilar Pierlé (1) , Odile Sismeiro (2) , Bernd Jagla (2) , Marie-Agnès Dillies (2) , Hugo Varet (2) , Oihane Irazoki (3) , Susana Campoy (3) , Zoé Rouy (4) , Stéphane Cruveiller (4) , Claudine Médigue (4) , Jean-Yves Coppée (2) , Didier Mazel (1)

1 PGB - Plasticité du Génome Bactérien - Bacterial Genome Plasticity
2 PF2 - Transcriptome et Epigénome
3 UAB - Universitat Autònoma de Barcelona
4 UMR 8030 - Génomique métabolique

The SOS response is an almost ubiquitous response of cells to genotoxic stresses. The full complement of genes in the SOS regulon for Vibrio species has only been addressed through bioinformatic analyses predicting LexA binding box consensus and in vitro validation. Here, we perform whole transcriptome sequencing from Vibrio cholerae treated with mitomycin C as an SOS inducer to characterize the SOS regulon and other pathways affected by this treatment.

Keywords: nrdB; ncRNA; DNA repair; SOS response; Transcriptome; nrdA

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Genomics [q-bio.GN]

Evelyne Krin, Sebastian Aguilar Pierlé, Odile Sismeiro, Bernd Jagla, Marie-Agnès Dillies, et al.. Expansion of the SOS regulon of Vibrio cholerae through extensive transcriptome analysis and experimental validation. BMC Genomics, 2018, 19 (1), pp.373. ⟨10.1186/s12864-018-4716-8⟩. ⟨pasteur-02562431⟩