HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

mRNA localization is linked to translation regulation in the Caenorhabditis elegans germ lineage

Abstract : Caenorhabditis elegans early embryos generate cell-specific transcriptomes despite lacking active transcription, thereby presenting an opportunity to study mechanisms of post-transcriptional regulatory control. We observed that some cell-specific mRNAs accumulate non-homogenously within cells, localizing to membranes, P granules (associated with progenitor germ cells in the P lineage) and P-bodies (associated with RNA processing). The subcellular distribution of transcripts differed in their dependence on 3'UTRs and RNA binding proteins, suggesting diverse regulatory mechanisms. Notably, we found strong but imperfect correlations between low translational status and P granule localization within the progenitor germ lineage. By uncoupling translation from mRNA localization, we untangled a long-standing question: Are mRNAs directed to P granules to be translationally repressed, or do they accumulate there as a consequence of this repression? We found that translational repression preceded P granule localization and could occur independently of it. Further, disruption of translation was sufficient to send homogenously distributed mRNAs to P granules. These results implicate transcriptional repression as a means to deliver essential maternal transcripts to the progenitor germ lineage for later translation.
Document type :
Journal articles
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-02559475
Contributor : Anne Lassailly-Bondaz Connect in order to contact the contributor
Submitted on : Monday, April 25, 2022 - 10:19:00 AM
Last modification on : Tuesday, April 26, 2022 - 3:36:58 AM

File

dev186817.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Dylan Parker, Lindsay Winkenbach, Samuel Boyson, Matthew Saxton, Camryn Daidone, et al.. mRNA localization is linked to translation regulation in the Caenorhabditis elegans germ lineage. Development (Cambridge, England), Company of Biologists, 2020, 147 (13), pp.dev186817. ⟨10.1242/dev.186817⟩. ⟨pasteur-02559475v2⟩

Share

Metrics

Record views

42

Files downloads

67