Dynamic Growth and Shrinkage of the Salmonella-Containing Vacuole Determines the Intracellular Pathogen Niche - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Cell Reports Année : 2019

Dynamic Growth and Shrinkage of the Salmonella-Containing Vacuole Determines the Intracellular Pathogen Niche

Audrey Salles

Résumé

Salmonella is a human and animal pathogen that causes gastro-enteric diseases. The key to Salmonella infection is its entry into intestinal epithelial cells, where the bacterium resides within a Salmonella-containing vacuole (SCV). Salmonella entry also induces the formation of empty macropinosomes, distinct from the SCV, in the vicinity of the entering bacteria. A few minutes after its formation, the SCV increases in size through fusions with the surrounding macropinosomes. Salmonella also induces membrane tubules that emanate from the SCV and lead to SCV shrinkage. Here, we show that these antipodal events are utilized by Salmonella to either establish a vacuolar niche or to be released into the cytosol by SCV rupture. We identify the molecular machinery underlying dynamic SCV growth and shrinkage. In particular, the SNARE proteins SNAP25 and STX4 participate in SCV inflation by fusion with macropinosomes. Thus, host compartment size control emerges as a pathogen strategy for intracellular niche regulation.
Fichier principal
Vignette du fichier
Dynamic Growth and Shrinkage of the Salmonella_Containing Vacuole Determines.pdf (6.83 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

pasteur-02557367 , version 1 (28-04-2020)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

Virginie Stévenin, Yuen-Yan Chang, Yoann Le Toquin, Magalie Duchateau, Quentin Giai Gianetto, et al.. Dynamic Growth and Shrinkage of the Salmonella-Containing Vacuole Determines the Intracellular Pathogen Niche. Cell Reports, 2019, 29 (12), pp.3958-3973.e7. ⟨10.1016/j.celrep.2019.11.049⟩. ⟨pasteur-02557367⟩
146 Consultations
132 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More