Skip to Main content Skip to Navigation
Journal articles

A coiled coil switch mediates cold sensing by the thermosensory protein DesK

Abstract : The thermosensor histidine kinase DesK from Bacil-lus subtilis senses changes in membrane fluidity initiating an adaptive response. Structural changes in DesK have been implicated in transmembrane signal-ing, but direct evidence is still lacking. On the basis of structure-guided mutagenesis, we now propose a mechanism of DesK-mediated signal sensing and transduction. The data indicate that stabilization/ destabilization of a 2-helix coiled coil, which connects the transmembrane sensory domain of DesK to its cytosolic catalytic region, is crucial to control its sig-naling state. Computational modeling and simulations reveal couplings between protein, water and membrane mechanics. We propose that membrane thickening is the main driving force for signal sensing and that it acts by inducing helix stretching and rotation prompting an asymmetric kinase-competent state. Overall, the known structural changes of the sensor kinase, as well as further dynamic rearrangements that we now predict, consistently link structure determinants to activity modulation.
Complete list of metadata

Cited literature [65 references]  Display  Hide  Download
Contributor : Alejandro Buschiazzo Connect in order to contact the contributor
Submitted on : Saturday, April 25, 2020 - 1:27:33 AM
Last modification on : Thursday, September 1, 2022 - 8:44:06 AM




Emilio Saita, Luciano A Abriata, Yi Ting Tsai, Felipe Trajtenberg, Thomas Lemmin, et al.. A coiled coil switch mediates cold sensing by the thermosensory protein DesK. Molecular Microbiology, Wiley, 2015, 98 (2), pp.258-271. ⟨10.1111/mmi.13118⟩. ⟨pasteur-02554124⟩



Record views