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, Synthesis to be reported elsewhere

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, E represents free enzyme, I-F the 2,3-difluorosialic acid, E-I the sialyl-enzyme, and I the sugar product. If k i > kreact, E-I accumulates and inactivation is observed

T. Cruzi, contains over 100 genes encoding for trans-sialidase, of which about half are catalytically inactive and contain the mutation Y342H

J. C. Gebler, D. E. Trimbur, R. A. Warren, R. Aebersold, M. Namchuck et al., Glu to Asp mutant of Agrobacterium -glucosidase underwent time-dependent inactivation in the presence of substrate via formation of a stable tyrosyl-glycoside, Biochemistry, vol.34, p.14547, 1995.