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Using Cryo-EM to Investigate Bacterial Secretion Systems

Abstract : Bacterial secretion systems are responsible for releasing macromolecules to the extracellular milieu or directly into other cells. These membrane complexes are associated with pathogenicity and bacterial fitness. Understanding of these large assemblies has exponentially increased in the last few years thanks to electron microscopy. In fact, a revolution in this field has led to breakthroughs in characterizing the structures of secretion systems and other macromolecular machineries so as to obtain high-resolution images of complexes that could not be crystallized. In this review, we give a brief overview of structural advancements in the understanding of secretion systems, fo-cusing in particular on cryo-electron microscopy, whether tomography or single-particle analysis. We describe how such techniques have contributed to knowledge of the mechanism of macromolecule secretion in bacteria and the impact they will have in the future.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-02550351
Contributor : Francesca Gubellini <>
Submitted on : Wednesday, April 22, 2020 - 10:22:35 AM
Last modification on : Friday, June 12, 2020 - 5:38:06 PM

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Chiara Rapisarda, Matteo Tassinari, Francesca Gubellini, Rémi Fronzes. Using Cryo-EM to Investigate Bacterial Secretion Systems. Annual Review of Microbiology, Annual Reviews, 2018, 72, pp.231-254. ⟨10.1146/annurev-micro-090817-062702⟩. ⟨pasteur-02550351⟩

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