Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2017

Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities

Résumé

Antigen-specific T-cells are highly variable, spanning potent antiviral efficacy and damaging auto-reactivity. In virus infections, identifying the most efficacious responses is critical to vaccine design. However, current methods depend on indirect measures or on ex vivo expanded CTL clones. We here describe a novel application of cytotoxic saporin-conjugated tetramers to kill antigen-specific T-cells without significant off-target effects. The relative efficacy of distinct antiviral CD8+ T-cell specificity can be directly assessed via antigen-specific CD8+ T-cell depletion. The utility of these reagents is demonstrated here in identifying the CD8+ T-cell specificity most effective in preventing HIV progression in HIV-infected HLA-B*27-positive immune controllers.
Fichier principal
Vignette du fichier
journal.pone.0184496.pdf (3.5 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

pasteur-02549950 , version 1 (21-04-2020)

Licence

Paternité

Identifiants

Citer

Ellen Leitman, Christine Palmer, Søren Buus, Fabian Chen, Lynn Riddell, et al.. Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities. PLoS ONE, 2017, 12 (10), pp.e0184496. ⟨10.1371/journal.pone.0184496⟩. ⟨pasteur-02549950⟩

Collections

PASTEUR
24 Consultations
71 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More