Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities

Ellen Leitman; Christine Palmer; Søren Buus; Fabian Chen; Lynn Riddell; Stuart Sims; Paul Klenerman; Asier Saez-Cirion; Bruce Walker; Paul Hess; Marcus Altfeld; Philippa Matthews; Philip Goulder

doi:10.1371/journal.pone.0184496

Journal articles

Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities

Ellen Leitman ^{1, 2} Christine Palmer ³ Søren Buus ⁴ Fabian Chen ⁵ Lynn Riddell ⁶ Stuart Sims ⁷ Paul Klenerman ^{8, 1} Asier Saez-Cirion ⁹ Bruce Walker ^{2, 10} Paul Hess ¹¹ Marcus Altfeld ² Philippa Matthews ^{1, 6, 8} Philip Goulder ^{1, 10, *}

* Corresponding author

1 University of Oxford [Oxford]

2 HMS - Harvard Medical School [Boston]

3 Ragon Institute of MGH, MIT and Harvard

4 KU - University of Copenhagen = Københavns Universitet

5 Royal Berkshire Hospital

6 NHS Foundation Trust [London]

7 UZH - University of Zürich [Zürich]

8 John Radcliffe Hospital [Oxford University Hospital]

9 HIV, Inflammation et persistance

10 UKZN - University of KwaZulu-Natal

11 NC State - North Carolina State University [Raleigh]

Abstract : Antigen-specific T-cells are highly variable, spanning potent antiviral efficacy and damaging auto-reactivity. In virus infections, identifying the most efficacious responses is critical to vaccine design. However, current methods depend on indirect measures or on ex vivo expanded CTL clones. We here describe a novel application of cytotoxic saporin-conjugated tetramers to kill antigen-specific T-cells without significant off-target effects. The relative efficacy of distinct antiviral CD8+ T-cell specificity can be directly assessed via antigen-specific CD8+ T-cell depletion. The utility of these reagents is demonstrated here in identifying the CD8+ T-cell specificity most effective in preventing HIV progression in HIV-infected HLA-B*27-positive immune controllers.

Document type :

Journal articles

Domain :

Life Sciences [q-bio]

Life Sciences [q-bio] / Immunology

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https://hal-pasteur.archives-ouvertes.fr/pasteur-02549950
Contributor : Asier Saez-Cirion <>
Submitted on : Tuesday, April 21, 2020 - 6:43:35 PM
Last modification on : Thursday, April 23, 2020 - 5:50:10 PM

Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities

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Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities

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