Abstract : Antigen-specific T-cells are highly variable, spanning potent antiviral efficacy and damaging auto-reactivity. In virus infections, identifying the most efficacious responses is critical to vaccine design. However, current methods depend on indirect measures or on ex vivo expanded CTL clones. We here describe a novel application of cytotoxic saporin-conjugated tetramers to kill antigen-specific T-cells without significant off-target effects. The relative efficacy of distinct antiviral CD8+ T-cell specificity can be directly assessed via antigen-specific CD8+ T-cell depletion. The utility of these reagents is demonstrated here in identifying the CD8+ T-cell specificity most effective in preventing HIV progression in HIV-infected HLA-B*27-positive immune controllers.
https://hal-pasteur.archives-ouvertes.fr/pasteur-02549950 Contributor : Asier Saez-CirionConnect in order to contact the contributor Submitted on : Tuesday, April 21, 2020 - 6:43:35 PM Last modification on : Friday, April 29, 2022 - 3:12:02 PM