, Cells were diluted in 0.5% solution of methylcellulose (MP Biomedicals, LLC) in PBS. The time lapse was recorded and exported at 14 fps
, Dark-field video microscopy of complemented Fiocruz LV2756 fcpA -/+ strain. Video was generated using a Zeiss AxioImager.M2 microscope outfitted with an
, AxioCam MRm camera and analyzed images using the AxioVision 4.8.2 software (Carl Zeiss Microscopy LLC). Cells were diluted in 0.5% solution of methylcellulose (MP Biomedicals, LLC) in PBS. The time lapse was recorded and exported at 14 fps
, Dark-field video microscopy of complemented Fiocruz L1-130 fcpA -/+ strain. Video was generated using a Zeiss AxioImager.M2 microscope outfitted with an
, AxioCam MRm camera and analyzed images using the AxioVision 4.8.2 software (Carl Zeiss Microscopy LLC). Cells were diluted in 0.5% solution of methylcellulose (MP Biomedicals, LLC) in PBS. The time lapse was recorded and exported at 14 fps
, Three-dimensional reconstruction of mutant Fiocruz L1-130 fcpA -strain, shown in Figure 4. Reconstruction of cells were segmented using 3D modeling software Amira (Visage Imaging)
, Three-dimensional reconstruction of complemented Fiocruz
, + strain, shown in Figure 4. Reconstruction of cells were segmented using 3D modeling software Amira (Visage Imaging), as described in Experimental Procedures