Fast, Sensitive and Specific Detection of Thailand orthohantavirus and its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay - Archive ouverte HAL Access content directly
Journal Articles Viruses Year : 2019

Fast, Sensitive and Specific Detection of Thailand orthohantavirus and its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay

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Abstract

Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.
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pasteur-02545958 , version 1 (17-04-2020)

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Attribution - CC BY 4.0

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Vololoniaina Raharinosy, Jean-Michel Heraud, Harinirina Aina Rabemananjara, Sandra Telfer, Danielle Aurore Doll Rakoto, et al.. Fast, Sensitive and Specific Detection of Thailand orthohantavirus and its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay. Viruses, 2019, 11 (8), pp.718. ⟨10.3390/v11080718⟩. ⟨pasteur-02545958⟩
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