Fast, Sensitive and Specific Detection of Thailand orthohantavirus and its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay
Abstract : Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.
https://hal-pasteur.archives-ouvertes.fr/pasteur-02545958 Contributor : Jean-Marc ReynesConnect in order to contact the contributor Submitted on : Friday, April 17, 2020 - 3:33:06 PM Last modification on : Thursday, April 7, 2022 - 10:10:26 AM