Fast, Sensitive and Specific Detection of Thailand orthohantavirus and its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay

Vololoniaina Raharinosy (1, 2) , Jean-Michel Heraud (1, 2) , Harinirina Aina Rabemananjara (1, 2) , Sandra Telfer (3, 4) , Danielle Aurore Doll Rakoto (2) , Claudia Filippone (1, 2) , Jean-Marc Reynes (5)

1 IPM - Unité de Virologie [Antananarivo, Madagascar]
2 Faculté des Sciences - Université d'Antananarivo
3 Unité Peste - Plague Unit [Antananarivo, Madagascar]
4 Institute of Biological and Environmental Sciences [Aberdeen]
5 UBIVE - Biologie des Infections Virales Émergentes - Biology of Emerging Viral Infections

Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.

Keywords: Hantavirus; Real-time RT-PCR; Thailand orthohantavirus

Domains: Life Sciences [q-bio]; Microbiology and Parasitology; Virology; Life Sciences [q-bio]; Human health and pathology; Infectious diseases

Vololoniaina Raharinosy, Jean-Michel Heraud, Harinirina Aina Rabemananjara, Sandra Telfer, Danielle Aurore Doll Rakoto, et al.. Fast, Sensitive and Specific Detection of Thailand orthohantavirus and its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay. Viruses, 2019, 11 (8), pp.718. ⟨10.3390/v11080718⟩. ⟨pasteur-02545958⟩