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Fast, Sensitive and Specific Detection of Thailand orthohantavirus and its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay

Vololoniaina Raharinosy 1, 2 Jean-Michel Heraud 1, 2, * Harinirina Aina Rabemananjara 1, 2 Sandra Telfer 3, 4 Danielle Aurore Doll Rakoto 2 Claudia Filippone 1, 2 Jean-Marc Reynes 5
* Corresponding author
1 IPM - Unité de Virologie [Antananarivo, Madagascar]
2 Faculté des Sciences - Université d'Antananarivo
3 Unité Peste - Plague Unit [Antananarivo, Madagascar]
4 Institute of Biological and Environmental Sciences [Aberdeen]
5 UBIVE - Biologie des Infections Virales Émergentes - Biology of Emerging Viral Infections
Abstract : Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.
Keywords : Thailand orthohantavirus Real-time RT-PCR Hantavirus
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Submitted on : Friday, April 17, 2020 - 3:33:06 PM
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Vololoniaina Raharinosy, Jean-Michel Heraud, Harinirina Aina Rabemananjara, Sandra Telfer, Danielle Aurore Doll Rakoto, et al.. Fast, Sensitive and Specific Detection of Thailand orthohantavirus and its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay. Viruses, MDPI, 2019, 11 (8), pp.718. ⟨10.3390/v11080718⟩. ⟨pasteur-02545958⟩

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