Skip to Main content Skip to Navigation
Journal articles

Neutrophil Extracellular Traps are Involved in the Innate Immune Response to Infection with Leptospira

Abstract : NETosis is a process by which neutrophils extrude their DNA together with bactericidal proteins that trap and/or kill pathogens. In the present study, we evaluated the ability of Leptospira spp. to induce NETosis using human ex vivo and murine in vivo models. Microscopy and fluorometric studies showed that incubation of human neutrophils with Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 (LIC) resulted in the release of DNA extracellular traps (NETs). The bacteria number, pathogenicity and viability were relevant factors for induction of NETs, but bacteria motility was not. Entrapment of LIC in the NETs resulted in LIC death; however, pathogenic but not saprophytic Leptospira sp. exerted nuclease activity and degraded DNA. Mice infected with LIC showed circulating NETs after 2 days post-infection (dpi). Depletion of neutrophils with mAb1A8 significantly reduced the amount of intravascular NETs in LIC-infected mice, increasing bacteremia at 3 dpi. Although there was a low bacterial burden, scarce neutrophils and an absence of inflammation in the early stages of infection in the kidney and liver, at the beginning of the leptospiruric phase, the bacterial burden was significantly higher in kidneys of neutrophil-depleted-mice compared to non-depleted and infected mice. Surprisingly, interstitial nephritis was of similar intensity in both groups of infected mice. Taken together, these data suggest that LIC triggers NETs, and that the intravascular formation of these DNA traps appears to be critical not only to prevent early leptospiral dissemination but also to preclude further bacterial burden.
Document type :
Journal articles
Complete list of metadatas

Cited literature [53 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-02538166
Contributor : Sylvie Murguet <>
Submitted on : Thursday, April 9, 2020 - 11:41:58 AM
Last modification on : Tuesday, May 26, 2020 - 4:22:03 AM

File

PMID 26161745 PLOS NEGL TROP D...
Files produced by the author(s)

Identifiers

Collections

Citation

Emilia Scharrig, Agostina Carestia, María Ferrer, Maia Cédola, Gabriela Pretre, et al.. Neutrophil Extracellular Traps are Involved in the Innate Immune Response to Infection with Leptospira. PLoS Neglected Tropical Diseases, Public Library of Science, 2015, 9 (7), pp.e0003927. ⟨10.1371/journal.pntd.0003927⟩. ⟨pasteur-02538166⟩

Share

Metrics

Record views

36

Files downloads

77