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B. Koehl, P. Nivoit, E. Nemer, and W. , The endothelin B receptor plays a crucial role in the adhesion of neutrophils to the endothelium in sickle cell disease, Haematologica, vol.102, issue.7, pp.1161-72, 2017.

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URL : https://hal.archives-ouvertes.fr/pasteur-01534181

, Flow Cytometry Expression of CD62L and CD64 on purified neutrophils was tested with APC Anti-Human CD62L Antibody (Biolegend -clone DREG-56) and PE Anti-Human CD64 Antibody

, SDS 2%, 1mM PMSF, Phosphatase Inhibitor Cocktail 3 (Sigma -ref P0044), complete EDTA-free (Roche -ref 11 873 580 001). The total protein concentration was determined by the BCA method (Pierce, Thermo Fisher Scientific) and 50 ?g of proteins were, Western Blot Analysis Purified neutrophils were lysed in lysis buffer containing 100 mM Tris/HCl (pH8.5), pp.4-15

, Bio-Rad) under reducing conditions. The gel was imaged using the stain-free application on the ChemiDoc MP (Bio-Rad) imager immediately after the protein separation in order to normalize the loading charge, SDS-PAGE using TGX Stain-Free Precast Gel

, anti-Human ISG15 (MAB 4845 -R&D System 0,5µg/ml), anti-Human Stat 1 (clone 10C4B40 -Biolegend 1µg/ml), anti-Human Stat2 (MAB1666 -R&D Systems 0,2µg/ml), anti-Human Phospho-Stat1 (MAB2894-R&D Systems 0,25µg/ml) and anti-Human Phospho-Stat2 (AF2890 -R&D Systems 0,5µg/ml). Immune complexes were revealed with secondary peroxidase-conjugated antibodies (Abliance) using a chemiluminescent kit, Blots were probed with anti-Human MX1 (HPA030917 -Sigma 0,4µg/ml), anti-Human IFIT1 (PA3-848 -ThermoFisher Scientific 1:2000)

, Quantification of Interferon alpha and beta at the protein level in plasma Whole blood was collected from SS patients at steady state and after centrifugation at 5 mn at 3000 rpm, plasma was collected and frozen at -20°C. Interferon alpha (IFN?) and Interferon beta (IFN?) protein level was quantified using a digital-ELISA assay (SIMOA, Quanterix) developped by Rodero et al 5 for the first dosage and the UMR INSERM 1223, p.4

, For IFN? quantification, the 8H1 antibody clone was used as a capture antibody, after coating on paramagnetic beads (0.3 mg/ml), and the 12H5 antibody was biotinylated (biotin-to-antibody ratio 30: 1) and used as the detector. Detector and revelation enzyme SBG concentrations were 0,3 ug/mL and 150 pM respectively. For IFN? quantification, a mouse anti-human IFN? Antibody (PBL Assay Science, Cat No. 710322-9), was used as a capture antibody

. Antibody, 710323-9) was biotinylated (biotin-to-antibody ratio 40: 1) and used as the detector. Detector and revelation enzyme SBG concentrations were 1

, PBL Assay Science) were used to generate a standard curve, after cross-reactivity testing. Each plasma sample was analyzed in duplicate and in dilution 1:6. The limit of detection (LOD) was calculated as the logarithmic mean value +2SD (95% confidence) of reactivity from all blank runs, and found to be 0.10 fg/mL for IFN? and 2.2 pg/mL for IFN?, ug/mL and 50 pM respectively. Recombinant IFN?17/?I (PBL Assay Science) and IFN?

E. F. Gautier, S. Ducamp, and M. Leduc, Comprehensive Proteomic Analysis of Human Erythropoiesis, Cell Rep, vol.16, issue.5, pp.1470-1484, 2016.
URL : https://hal.archives-ouvertes.fr/inserm-01356018

N. A. Kulak, G. Pichler, I. Paron, N. Nagaraj, and M. Mann, Minimal, encapsulated proteomicsample processing applied to copy-number estimation in eukaryotic cells, Nat Methods, vol.11, issue.3, pp.319-324, 2014.

J. T. Cox, I. Marginean, R. T. Kelly, R. D. Smith, and K. Tang, Improving the sensitivity of mass spectrometry by using a new sheath flow electrospray emitter array at subambient pressures, J Am Soc Mass Spectrom, vol.25, issue.12, pp.2028-2037, 2014.

B. Koehl, P. Nivoit, E. Nemer, and W. , The endothelin B receptor plays a crucial role in the adhesion of neutrophils to the endothelium in sickle cell disease, Haematologica, vol.102, issue.7, pp.1161-72, 2017.

M. P. Rodero, J. Decalf, and V. Bondet, Detection of interferon alpha protein reveals differential levels and cellular sources in disease, J Exp Med, vol.214, issue.5, pp.1547-1555, 2017.
URL : https://hal.archives-ouvertes.fr/pasteur-01534181

, Age

, Reticulocyte count (/mm 3 ) 297,370, vol.157, pp.800-329