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Article Dans Une Revue Vaccine Année : 2020

Subcutaneous vaccination with a live attenuated Yersinia pseudotuberculosis plague vaccine

Anne Derbise
Chloé Guillas
  • Fonction : Auteur
Elisabeth Carniel
  • Fonction : Auteur
  • PersonId : 1039366
Javier Pizarro-Cerdá
Christian E. Demeure

Résumé

A single oral inoculation to mice of the live attenuated Yersinia pseudotuberculosis VTnF1 strain producing an F1 pseudocapsule protects against bubonic and pneumonic plague. However oral vaccination can fail in humans exposed to frequent intestinal infections. We evaluated in mice the efficacy of subcutaneous vaccine injection as an alternative way to induce protective immunity, while reducing the dose and avoiding strain release in nature. A single subcutaneous dose of up to 108 CFU induced dose-dependent antibody production. At the dose of 107 CFU, i.e. 10 times less than via the oral route, it caused a modest skin reaction and protected 100% against bubonic and 80% against pneumonic plague, caused by high doses of Yersinia pestis. Bacteria migrating to lymph nodes and spleen, but not feces, were rapidly eliminated. Thus, subcutaneous injection of VTnF1 would represent a good alternative when dissemination in nature and human intestinal responsiveness are limitations.
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Dates et versions

pasteur-02493687 , version 1 (28-02-2020)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

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Citer

Anne Derbise, Chloé Guillas, Christiane Gerke, Elisabeth Carniel, Javier Pizarro-Cerdá, et al.. Subcutaneous vaccination with a live attenuated Yersinia pseudotuberculosis plague vaccine. Vaccine, 2020, 38 (8), pp.1888-1892. ⟨10.1016/j.vaccine.2020.01.014⟩. ⟨pasteur-02493687⟩

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