Skip to Main content Skip to Navigation
Journal articles

Subcutaneous vaccination with a live attenuated Yersinia pseudotuberculosis plague vaccine

Abstract : A single oral inoculation to mice of the live attenuated Yersinia pseudotuberculosis VTnF1 strain producing an F1 pseudocapsule protects against bubonic and pneumonic plague. However oral vaccination can fail in humans exposed to frequent intestinal infections. We evaluated in mice the efficacy of subcutaneous vaccine injection as an alternative way to induce protective immunity, while reducing the dose and avoiding strain release in nature. A single subcutaneous dose of up to 108 CFU induced dose-dependent antibody production. At the dose of 107 CFU, i.e. 10 times less than via the oral route, it caused a modest skin reaction and protected 100% against bubonic and 80% against pneumonic plague, caused by high doses of Yersinia pestis. Bacteria migrating to lymph nodes and spleen, but not feces, were rapidly eliminated. Thus, subcutaneous injection of VTnF1 would represent a good alternative when dissemination in nature and human intestinal responsiveness are limitations.
Complete list of metadatas

Cited literature [24 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-02493687
Contributor : Christian E. Demeure <>
Submitted on : Friday, February 28, 2020 - 9:19:47 AM
Last modification on : Thursday, March 5, 2020 - 4:25:48 PM
Long-term archiving on: : Friday, May 29, 2020 - 1:22:26 PM

File

2020 Derbise JVAC.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License

Identifiers

Collections

Citation

Anne Derbise, Chloé Guillas, Christiane Gerke, Elisabeth Carniel, Javier Pizarro-Cerdá, et al.. Subcutaneous vaccination with a live attenuated Yersinia pseudotuberculosis plague vaccine. Vaccine, Elsevier, 2020, 38 (8), pp.1888-1892. ⟨10.1016/j.vaccine.2020.01.014⟩. ⟨pasteur-02493687⟩

Share

Metrics

Record views

156

Files downloads

219