Skip to Main content Skip to Navigation
New interface
Journal articles

Targeting and crossing of the human maternofetal barrier by Listeria monocytogenes: Role of internalin interaction with trophoblast E-cadherin

Abstract : Listeria monocytogenes produces severe fetoplacental infections in humans. How it targets and crosses the maternofetal barrier is unknown. We used immunohistochemistry to examine the location of L. monocytogenes in placental and amniotic tissue samples obtained from women with fetoplacental listeriosis. The results raised the possibility that L. monocytogenes crosses the ma-ternofetal barrier through the villous syncytiotrophoblast, with secondary infection occurring via the amniotic epithelium. Because epidemiological studies indicate that the bacterial surface protein, internalin (InlA), may play a role in human fetoplacental listeriosis, we investigated the cellular patterns of expression of its host receptor, E-cadherin, at the maternofetal interface. E-cadherin was found on the basal and apical plasma membranes of syncytiotro-phoblasts and in villous cytotrophoblasts. Established trophoblas-tic cell lines, primary trophoblast cultures, and placental villous explants were each exposed to isogenic InlA؉ or InlA؊ strains of L. monocytogenes, and to L. innocua expressing or not InlA. Quantitative assays of cellular invasion demonstrated that bacterial entry into syncytiotrophoblasts occurs via the apical membrane in an InlA-E-cadherin dependent manner. In human placental villous explants, bacterial invasion of the syncytiotrophoblast barrier and underlying villous tissue and subsequent replication produces histopathological lesions that mimic those seen in pla-centas of women with listeriosis. Thus, the InlA-E-cadherin interaction that plays a key role in the crossing of the intestinal barrier in humans is also exploited by L. monocytogenes to target and cross the placental barrier. Such a ligand-receptor interaction allowing a pathogen to specifically cross the placental villous trophoblast barrier has not been reported previously.
Complete list of metadata

Cited literature [34 references]  Display  Hide  Download
Contributor : andrée DIAKITE Connect in order to contact the contributor
Submitted on : Monday, June 1, 2020 - 2:10:07 AM
Last modification on : Tuesday, May 31, 2022 - 10:20:16 AM
Long-term archiving on: : Thursday, September 24, 2020 - 7:57:19 PM


2004_Lecuit_Proceedings of the...
Publisher files allowed on an open archive


Distributed under a Creative Commons Attribution - ShareAlike 4.0 International License




Marc Lecuit, D Michael Michael Nelson, Steve D Smith, Huot Khun, Michel Huerre, et al.. Targeting and crossing of the human maternofetal barrier by Listeria monocytogenes: Role of internalin interaction with trophoblast E-cadherin. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101 (16), pp.6152-6157. ⟨10.1073/pnas.0401434101⟩. ⟨pasteur-02456710⟩



Record views


Files downloads