Mouse Models and Tools for the in vivo Study of Neutrophils

Julien Stackowicz (1, 2) , Friederike Jönsson (1) , Laurent L. Reber (1, 3)

1 Anticorps en thérapie et pathologie - Antibodies in Therapy and Pathology
2 Collège Doctoral
3 CPTP - Centre de Physiopathologie Toulouse Purpan

Neutrophils are the most abundant leukocytes in human blood and critical actors of the immune system. Many neutrophil functions and facets of their activity in vivo were revealed by studying genetically modified mice or by tracking fluorescent neutrophils in animals using imaging approaches. Assessing the roles of neutrophils can be challenging, especially when exact molecular pathways are questioned or disease states are interrogated that alter normal neutrophil homeostasis. This review discusses the main in vivo models for the study of neutrophils, their advantages and limitations. The side-by-side comparison underlines the necessity to carefully choose the right model(s) to answer a given scientific question, and exhibit caveats that need to be taken into account when designing experimental procedures. Collectively, this review suggests that at least two models should be employed to legitimately conclude on neutrophil functions.

Mots clés: neutrophils; Cre-recombinase; diphtheria toxin; mouse models; depletion; Gfi-1; NETosis

Domaines: Immunologie

Julien Stackowicz, Friederike Jönsson, Laurent L. Reber. Mouse Models and Tools for the in vivo Study of Neutrophils. Frontiers in Immunology, 2020, 10, pp.3130. ⟨10.3389/fimmu.2019.03130⟩. ⟨pasteur-02447221⟩