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Journal articles
Effect of nuclear architecture on the efficiency of double-strand break repair
Abstract : The most dangerous insults to the genome's integrity are those that break both strands of the DNA. Double-strand breaks can be repaired by homologous recombination; in this conserved mechanism, a global genomic homology search finds sequences similar to those near the break, and uses them as a template for DNA synthesis and ligation. Chromosomes occupy restricted territories within the nucleus. We show that yeast genomic regions whose nuclear territories overlap recombine more efficiently than sequences located in spatially distant territories. Tethering of telomeres and centromeres reduces the efficiency of recombination between distant genomic loci, lowering the chances of non-allelic recombination. Our results challenge present models that posit an active scanning of the whole nuclear volume by the broken chromosomal end; they demonstrate that the search for homology is a limiting step in homologous recombination, and emphasize the importance of nuclear organization in genome maintenance.
Cited literature [36 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-02446715
Contributor : Marie Lemesle <>
Submitted on : Tuesday, January 21, 2020 - 10:01:10 AM
Last modification on : Friday, September 4, 2020 - 12:08:01 PM
Contributor : Marie Lemesle <>
Submitted on : Tuesday, January 21, 2020 - 10:01:10 AM
Last modification on : Friday, September 4, 2020 - 12:08:01 PM