Skip to Main content Skip to Navigation
Journal articles

A sensitive fluorescence-based assay to monitor enzymatic activity of the essential integral membrane protein Apolipoprotein N-acyltransferase (Lnt)

Abstract : Lipoprotein modification is an essential process in Gram-negative bacteria. The action of three integral membrane proteins that catalyze the transfer of fatty acids derived from membrane phospholipids or cleave the signal peptide of the lipoprotein substrate result in the formation of mature triacylated proteins. Inactivation of the enzymes leads to mis-localization of immature lipoproteins and consequently cell death. Biochemical studies and the development of in vitro assays are challenging due to the fact that the enzymes and substrates are all membrane-embedded proteins difficult to overproduce and purify. Here we describe a sensitive fluorescence-based assay to monitor bacterial apolipoprotein N-acyltransferase activity.
Complete list of metadatas

Cited literature [24 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-02421050
Contributor : Nienke Buddelmeijer <>
Submitted on : Friday, December 20, 2019 - 11:29:43 AM
Last modification on : Wednesday, May 6, 2020 - 5:16:02 PM
Long-term archiving on: : Saturday, March 21, 2020 - 3:21:00 PM

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Karine Nozeret, Alix Boucharlat, Fabrice Agou, Nienke Buddelmeijer. A sensitive fluorescence-based assay to monitor enzymatic activity of the essential integral membrane protein Apolipoprotein N-acyltransferase (Lnt). Scientific Reports, Nature Publishing Group, 2019, 9 (1), pp.15978. ⟨10.1038/s41598-019-52106-8⟩. ⟨pasteur-02421050⟩

Share

Metrics

Record views

168

Files downloads

187