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Journal articles
Dual activity of PNGM-1 pinpoints the evolutionary origin of subclass B3 metallo- β -lactamases: a molecular and evolutionary study
Abstract : Resistance to β-lactams is one of the most serious problems associated with Gram-negative infections. β-Lactamases are able to hydrolyze β-lactams such as cephalosporins and/or carbapenems. Evolutionary origin of metallo-β-lactamases (MBLs), conferring critical antibiotic resistance threats, remains unknown. We discovered PNGM-1, the novel subclass B3 MBL, in deep-sea sediments that predate the antibiotic era. Here, our phylogenetic analysis suggests that PNGM-1 yields insights into the evolutionary origin of subclass B3 MBLs. We reveal the structural similarities between tRNase Zs and PNGM-1, and demonstrate that PNGM-1 has both MBL and tRNase Z activities, suggesting that PNGM-1 is thought to have evolved from a tRNase Z. We also show kinetic and structural comparisons between PNGM-1 and other proteins including subclass B3 MBLs and tRNase Zs. These comparisons revealed that the B3 MBL activity of PNGM-1 is a promiscuous activity and subclass B3 MBLs are thought to have evolved through PNGM-1 activity.
Cited literature [42 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-02399571
Contributor : Sang Hee Lee <>
Submitted on : Monday, December 9, 2019 - 10:41:53 AM
Last modification on : Friday, December 13, 2019 - 2:38:28 AM
Long-term archiving on: : Tuesday, March 10, 2020 - 2:50:52 PM
Contributor : Sang Hee Lee <>
Submitted on : Monday, December 9, 2019 - 10:41:53 AM
Last modification on : Friday, December 13, 2019 - 2:38:28 AM
Long-term archiving on: : Tuesday, March 10, 2020 - 2:50:52 PM
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Dual activity of PNGM-1_EMI-20...
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