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Intérêt de la biologie moléculaire dans le diagnostic des infections vlrales du système nerveux central

Abstract : The use of PCR and hybridization for the detection of viral nucleic acids in cerebrospinal fluid (CSF) currently represents the most reliable method for the meningoencephalitis diagnosis due to herpes viruses, enteroviruses, JC polyomavirus and HIV-1 primary infection. The molecular tools are less suitable for the postinfectious encephalitis associated with a systemic viral infection such as neurologic complications of measles, rubella, influenza and varicella, The speed and high sensitivity allow earlier diagnosis and specific treatment. The monitoring of the antiviral therapy is assessed by the development of quantitative amplification assays in CSF or by a negative PCR result. The pattern mutations conferring resistance and the genetic variability of viruses (HIV-1, Dengue virus) could be determined from different locations in the genome. Theses methods performed over the past several years as research tools have now a large scale application with the commercialization of some tests. But before becoming the first line diagnostic test, PCR of CSF could be conducted with control quality and evaluated protocols for avoiding false positive and negative results.
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Contributor : Vincent Lacoste <>
Submitted on : Friday, December 6, 2019 - 5:21:54 PM
Last modification on : Thursday, December 12, 2019 - 1:02:47 AM

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Elisabeth Nicand, Vincent Lacoste, Marc Grandadam, Remy Teyssou, Yves Buisson. Intérêt de la biologie moléculaire dans le diagnostic des infections vlrales du système nerveux central. Revue Française des Laboratoires, Elsevier Masson SAS, 1999, 1999 (315), pp.55-62. ⟨10.1016/S0338-9898(99)80488-0⟩. ⟨pasteur-02397899⟩

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