Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes

Viviane Chenal-Francisque; Mylène M Maury; Morgane Lavina; Marie Touchon; Alexandre Leclercq; Marc Lecuit; Sylvain Brisse

doi:10.1128/JCM.00738-15

Journal articles

Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes

Viviane Chenal-Francisque ^{1, 2} Mylène M Maury ^{3, 4} Morgane Lavina ^{1, 2} Marie Touchon ⁴ Alexandre Leclercq ^{1, 2} Marc Lecuit ^{5, 6, 7, 8, 9, 2, *} Sylvain Brisse ^{4, *}

* Corresponding author

1 CNRL - Centre National de Référence Listeria - National Reference Center Listeria

2 CC-OMS / WHO-CC - Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria

3 Cellule Pasteur

4 Génomique évolutive des Microbes / Microbial Evolutionary Genomics

5 Biologie des Infections - Biology of Infection

6 UPD5 - Université Paris Descartes - Paris 5

7 CHU Necker - Enfants Malades [AP-HP]

8 Service des Maladies infectieuses et tropicales [CHU Necker]

9 Imagine - U1163 - Imagine - Institut des maladies génétiques (IHU)

Abstract : Three multiplex PCR assays were developed to identify the 11 most common Listeria monocytogenes clones in clinical and food samples; 270 (95.7%) of 282 strains of serogroups IVb, IIb, IIa, and IIc were identified accurately. This novel tool is a rapid and efficient alternative to multilocus sequence typing for identification of L. monocytogenes clones.

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Journal articles

Domain :

Life Sciences [q-bio] / Human health and pathology

Life Sciences [q-bio] / Human health and pathology / Infectious diseases

Life Sciences [q-bio] / Microbiology and Parasitology

Life Sciences [q-bio] / Microbiology and Parasitology / Bacteriology

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Submitted on : Tuesday, December 3, 2019 - 1:27:08 PM
Last modification on : Thursday, October 20, 2022 - 3:10:07 PM

Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes

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Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes

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