Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes

Viviane Chenal-Francisque (1, 2) , Mylène M Maury (3, 4) , Morgane Lavina (1, 2) , Marie Touchon (4) , Alexandre Leclercq (1, 2) , Marc Lecuit (5, 6, 7, 8, 9, 2) , Sylvain Brisse (4)

1 CNRL - Centre National de Référence Listeria - National Reference Center Listeria
2 CC-OMS / WHO-CC - Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria
3 Cellule Pasteur
4 Génomique évolutive des Microbes / Microbial Evolutionary Genomics
5 Biologie des Infections - Biology of Infection
6 UPD5 - Université Paris Descartes - Paris 5
7 Hôpital Necker - Enfants Malades [AP-HP]
8 Service des Maladies infectieuses et tropicales [CHU Necker]
9 Imagine - U1163 - Imagine - Institut des maladies génétiques (IHU)

Three multiplex PCR assays were developed to identify the 11 most common Listeria monocytogenes clones in clinical and food samples; 270 (95.7%) of 282 strains of serogroups IVb, IIb, IIa, and IIc were identified accurately. This novel tool is a rapid and efficient alternative to multilocus sequence typing for identification of L. monocytogenes clones.

Domaines: Médecine humaine et pathologie; Maladies infectieuses; Microbiologie et Parasitologie; Bactériologie

Viviane Chenal-Francisque, Mylène M Maury, Morgane Lavina, Marie Touchon, Alexandre Leclercq, et al.. Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes. Journal of Clinical Microbiology, 2015, 53 (10), pp.3355-3358. ⟨10.1128/JCM.00738-15⟩. ⟨pasteur-02390981⟩