Insights into the Host Range, Genetic Diversity, and Geographical Distribution of Jingmenviruses

Sarah Temmam; Thomas Bigot; Delphine Chrétien; Mathilde Gondard; Philippe Pérot; Virginie Pommelet; Evelyne Dufour; Stéphane Petres; Elodie Devillers; Thavry Hoem; Valérie Pinarello; Vibol Hul; Khamsing Vongphayloth; Jeffrey Hertz; Irène Loiseau; Marine Dumarest; Veasna Duong; Muriel Vayssier-Taussat; Marc Grandadam; Emmanuel Albina; Philippe Dussart; Sara Moutailler; Julien Cappelle; Paul Brey; Marc Eloit

doi:10.1128/mSphere.00645-19

Journal articles

Insights into the Host Range, Genetic Diversity, and Geographical Distribution of Jingmenviruses

Sarah Temmam ¹ Thomas Bigot ^{1, 2} Delphine Chrétien ¹ Mathilde Gondard ^{3, 4} Philippe Pérot ¹ Virginie Pommelet ⁵ Evelyne Dufour ⁶ Stéphane Petres ⁶ Elodie Devillers ⁴ Thavry Hoem ⁷ Valérie Pinarello ³ Vibol Hul ⁷ Khamsing Vongphayloth ⁵ Jeffrey Hertz ⁸ Irène Loiseau ¹ Marine Dumarest ¹ Veasna Duong ⁷ Muriel Vayssier-Taussat ⁴ Marc Grandadam ⁵ Emmanuel Albina ³ Philippe Dussart ⁷ Sara Moutailler ⁴ Julien Cappelle ^{3, 7, 9} Paul Brey ⁵ Marc Eloit ^{1, 10, 11, *}

* Corresponding author

1 Découverte de Pathogènes - Pathogen Discovery

Biologie des Infections - Biology of Infection

2 Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB

3 UMR ASTRE - Animal, Santé, Territoires, Risques et Ecosystèmes

4 BIPAR - Biologie moléculaire et immunologie parasitaires et fongiques

5 Institut Pasteur du Laos

6 Plateforme technologique Production et purification de protéines recombinantes – Production and Purification of Recombinant Proteins Technological Platform

7 Institut Pasteur du Cambodge

8 NAMRU-2 - Naval Medical Research Unit Two [Cambodge]

9 UMR EPIA - Unité Mixte de Recherche d'Épidémiologie des maladies Animales et zoonotiques

10 ENVA - École nationale vétérinaire d'Alfort

11 UPE - Université Paris-Est

Abstract : Jingmenvirus is a recently identified group of segmented RNA viruses phylogenetically linked with unsegmented Flaviviridae viruses. Primarily identified in various tick genera originating in China, Jingmenvirus geographical distribution has rapidly expanded to cover Africa, South America, Caribbean, and Europe. The identification of Jingmen-related viruses in various mammals, including febrile humans, opens the possibility that Jingmenviruses may be novel tick-borne arboviruses. In this study, we aimed at increasing knowledge of the host range, genetic diversity, and geographical distribution of Jingmenviruses by reporting for the first time the identification of Jingmenviruses associated with Rhipicephalus microplus ticks originating in the French Antilles (Guadeloupe and Martinique islands), with Amblyomma testudinarium ticks in Lao PDR, and with Ixodes ricinus ticks in metropolitan France, and from urine of Pteropus lylei bats in Cambodia. Analyses of the relationships between the different Jingmenvirus genomes resulted in the identification of three main phylogenic subclades, each of them containing both tick-borne and mammal-borne strains, reinforcing the idea that Jingmenviruses may be considered as tick-borne arboviruses. Finally, we estimated the prevalence of Jingmenvirus-like infection using luciferase immunoprecipitation assay screening (LIPS) of asymptomatic humans and cattle highly exposed to tick bites. Among 70 French human, 153 Laotian human, and 200 Caribbean cattle sera tested, only one French human serum was found (slightly) positive, suggesting that the prevalence of Jingmenvirus human and cattle infections in these areas is probably low.

Keywords : Jingmenvirus LIPS emergence evolution

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Microbiology and Parasitology / Virology

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Insights into the Host Range, Genetic Diversity, and Geographical Distribution of Jingmenviruses

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Insights into the Host Range, Genetic Diversity, and Geographical Distribution of Jingmenviruses

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