, Gram-negative-type PG and Gram-positive meso-DAPNH 2 -type PG

B. Buffer,

, Other Gram-positive-type PG: Use a 0-100 % buffer B linear gradient over 280 min

, Buffer A(G+): 10 mM NH 4 PO 4 pH 5

B. Buffer and . G+, 10 mM NH 4 PO 4 pH 5.6 with 30 %. methanol

, For desalting muropeptides (prior to mass spectrometry analysis): Use a 0-50 % buffer B linear gradient over 30 min, Buffer A(Ds): 0.05 % TFA

, Buffer B(Ds): 0.05 % TFA with 50 % acetonitrile

, Protocol IV: Verification of the Absence of SDS in PG Sample According to Hayashi et al. (1975) Stock buffers, solutions

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