Skip to Main content Skip to Navigation
Journal articles

CotL, a new morphogenetic spore coat protein of Clostridium difficile

Abstract : The strict anaerobe Clostridium difficile is the most common cause of antibiotic-associated diarrhoea. The oxygen-resistant C. difficile spores play a central role in the infectious cycle, contributing to transmission, infection and recurrence. The spore surface layers, the coat and exosporium, enable the spores to resist physical and chemical stress. However, little is known about the mechanisms of their assembly. In this study, we characterized a new spore protein, CotL, which is required for the assembly of the spore coat. The cotL gene was expressed in the mother cell compartment under the dual control of the RNA polymerase sigma factors, σE and σK . CotL was localized in the spore coat, and the spores of the cotL mutant had a major morphologic defect at the level of the coat/exosporium layers. Therefore, the mutant spores contained a reduced amount of several coat/exosporium proteins and a defect in their localization in sporulating cells. Finally, cotL mutant spores were more sensitive to lysozyme and were impaired in germination, a phenotype likely to be associated with the structurally altered coat. Collectively, these results strongly suggest that CotL is a morphogenetic protein essential for the assembly of the spore coat in C. difficile.
Document type :
Journal articles
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-02331056
Contributor : Virginie Portelette <>
Submitted on : Thursday, October 24, 2019 - 11:36:45 AM
Last modification on : Monday, May 11, 2020 - 10:38:03 PM

Identifiers

Citation

Carolina Alves Feliciano, Thibaut Douche, Quentin Giai Gianetto, Mariette Matondo, Isabelle Martin-Verstraete, et al.. CotL, a new morphogenetic spore coat protein of Clostridium difficile. Environmental Microbiology, Wiley-Blackwell, 2019, 21 (3), pp.984-1003. ⟨10.1111/1462-2920.14505⟩. ⟨pasteur-02331056⟩

Share

Metrics

Record views

297