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Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

Abstract : One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.
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Contributor : Virginie Portelette <>
Submitted on : Thursday, October 24, 2019 - 11:26:23 AM
Last modification on : Monday, January 13, 2020 - 5:08:19 PM

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Distributed under a Creative Commons Attribution 4.0 International License

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Daniel Donnelly, Catherine Rawlins, Caroline Dehart, Luca Fornelli, Luis Schachner, et al.. Best practices and benchmarks for intact protein analysis for top-down mass spectrometry. Nature Methods, Nature Publishing Group, 2019, 16 (7), pp.587-594. ⟨10.1038/s41592-019-0457-0⟩. ⟨pasteur-02331018⟩

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