Best practices and benchmarks for intact protein analysis for top-down mass spectrometry - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Nature Methods Année : 2019

Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

Jeremy Wolff
  • Fonction : Auteur
Paul Danis
  • Fonction : Auteur
Yury Tsybin
  • Fonction : Auteur

Résumé

One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.
Fichier principal
Vignette du fichier
s41592-019-0457-0.pdf (1.52 Mo) Télécharger le fichier
Loading...

Dates et versions

pasteur-02331018 , version 1 (24-10-2019)

Licence

Paternité

Identifiants

Citer

Daniel Donnelly, Catherine Rawlins, Caroline Dehart, Luca Fornelli, Luis Schachner, et al.. Best practices and benchmarks for intact protein analysis for top-down mass spectrometry. Nature Methods, 2019, 16 (7), pp.587-594. ⟨10.1038/s41592-019-0457-0⟩. ⟨pasteur-02331018⟩
77 Consultations
167 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More