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Journal articles
Single-site phosphorylation within the His-tag sequence attached to a recombinant protein
Abstract : We report the observation of single-site phosphorylation in a His-tag sequence N-terminally attached to a recombinant protein (UVI31+) in vitro. This modification was detected at position 23 at a serine residue of the His-tag sequence encoded by the vector pET28a. Furthermore, the phosphorylated tag sequence was found to be dephosphorylated by the action of alkaline phosphatases. The functional activity and dynamics of the protein carrying the His-tag sequence were unchanged after phosphorylation. The possibility of phosphorylation within the N-terminal His-tag demonstrates that care has to be taken upon analysis of post-translational modifications via mass spectrometry for recombinant protein expression strategies.
https://hal-pasteur.archives-ouvertes.fr/pasteur-02329972
Contributor : Benjamin Bardiaux Connect in order to contact the contributor
Submitted on : Wednesday, October 23, 2019 - 5:38:35 PM
Last modification on : Wednesday, November 3, 2021 - 9:32:44 AM
Contributor : Benjamin Bardiaux Connect in order to contact the contributor
Submitted on : Wednesday, October 23, 2019 - 5:38:35 PM
Last modification on : Wednesday, November 3, 2021 - 9:32:44 AM