Skip to Main content Skip to Navigation
Journal articles

Uncovering Listeria monocytogenes hypervirulence by harnessing its biodiversity

Mylène Maury 1, 2 Yu-Huan Tsai 3 Caroline Charlier 4, 5, 6, 7, 3 Marie Touchon 2 Viviane Chenal-Francisque 3, 7, 4 Alexandre Leclercq 7, 4, 3 Alexis Criscuolo 8 Charlotte Gaultier 3 Sophie Roussel 9, 10 Anne Brisabois 9, 10 Olivier Disson 3 Eduardo Rocha 2 Sylvain Brisse 2, * Marc Lecuit 4, 5, 11, 6, 7, *
* Corresponding author
1 Cellule Pasteur
2 Génomique évolutive des Microbes / Microbial Evolutionary Genomics
3 Biologie des Infections - Biology of Infection
4 CC-OMS / WHO-CC - Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria
5 UPD5 - Université Paris Descartes - Paris 5
6 Imagine - U1163 - Imagine - Institut des maladies génétiques (IHU)
7 CNRL - Centre National de Référence Listeria - National Reference Center Listeria
8 C3BI - Centre de Bioinformatique, Biostatistique et Biologie Intégrative
9 ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail
10 UPE - Université Paris-Est
11 CHU Necker - Enfants Malades [AP-HP]
Abstract : Microbial pathogenesis studies are typically performed with reference strains, thereby overlooking within-species heterogeneity in microbial virulence. Here we integrated human epidemiological and clinical data with bacterial population genomics to harness the biodiversity of the model foodborne pathogen Listeria monocytogenes and decipher the basis of its neural and placental tropisms. Taking advantage of the clonal structure of this bacterial species, we identify clones epidemiologically associated either with food or with human central nervous system (CNS) or maternal-neonatal (MN) listeriosis. The latter clones are also most prevalent in patients without immunosuppressive comorbidities. Strikingly, CNS- and MN-associated clones are hypervirulent in a humanized mouse model of listeriosis. By integrating epidemiological data and comparative genomics, we have uncovered multiple new putative virulence factors and demonstrate experimentally the contribution of the first gene cluster mediating L. monocytogenes neural and placental tropisms. This study illustrates the exceptional power in harnessing microbial biodiversity to identify clinically relevant microbial virulence attributes
Document type :
Journal articles
Complete list of metadata

Cited literature [57 references]  Display  Hide  Download
https://hal-pasteur.archives-ouvertes.fr/pasteur-02170775
Contributor : Andrée Diakite Connect in order to contact the contributor
Submitted on : Tuesday, July 2, 2019 - 1:34:16 PM
Last modification on : Thursday, November 25, 2021 - 4:18:04 PM

Links full text

pubmedcentral

Identifiers

Collections

PASTEUR | ANSES | CNRS | UNIV-PARIS5 | UNIV-PARIS7 | USPC | INSERM | UNIV-PARIS | ANR | UP-SANTE

Citation

Mylène Maury, Yu-Huan Tsai, Caroline Charlier, Marie Touchon, Viviane Chenal-Francisque, et al.. Uncovering Listeria monocytogenes hypervirulence by harnessing its biodiversity. Nature Genetics, Nature Publishing Group, 2016, 48 (3), pp.308-313. ⟨10.1038/ng.3501⟩. ⟨pasteur-02170775⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Les métriques sont temporairement indisponibles