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Single-cell transcriptional analysis reveals naïve helper ILC-like cells in zebrafish

Abstract : Innate lymphoid cells (ILCs) are important mediators of the immune response and homeostasis in barrier tissues of mammals. However, the existence and function of ILCs in other vertebrates is poorly understood. Here, we use single-cell RNA sequencing to generate a comprehensive atlas of zebrafish lymphocytes during tissue homeostasis and following immune challenge. We profiled 14,080 individual cells from the gut of wild-type zebrafish, as well as of rag1-deficient fish which lack T and B cells, and discovered diverse populations of helper ILC-like cells. Unexpectedly, fish displayed a rorc-positive, naïve subset that established a Type 3 or Type 2 response only upon immune challenge. Specifically, naïve ILC-like cells expressed il22 and tnfa following exposure to inactivated bacteria, or il13 following exposure to helminth extract. Cytokine-producing ILC-like cells express a specific repertoire of novel immune-type receptors, likely involved in recognition of environmental cues. We identified additional novel markers of zebrafish ILCs and generated a cloud repository for their in-depth exploration.
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Preprints, Working Papers, ...
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Contributor : Pedro Hernandez Connect in order to contact the contributor
Submitted on : Friday, May 31, 2019 - 10:22:34 AM
Last modification on : Tuesday, August 30, 2022 - 4:20:05 PM


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Pedro P Hernández, Paulina M Strzelecka, Emmanouil I Athanasiadis, Ana F Robalo, Catherine Collins, et al.. Single-cell transcriptional analysis reveals naïve helper ILC-like cells in zebrafish. 2019. ⟨pasteur-02144629⟩



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