Viral Small RNA Cloning and Sequencing

Valérie Gausson (1) , Maria-Carla P Saleh (1)

1 Virus et Interférence ARN

At the current rate of technological progress, high-throughput sequencing of nucleic acids has become a commodity. These techniques are perfectly suitable for viral small RNAs sequencing and contribute to the understanding of many aspects of virus biology in the context of host-pathogen interaction. However, the generation of high quality data is still an issue and the preparation of small RNAs libraries that accurately reflect the viral siRNAs in the sample remains a challenge. In this chapter we describe how to clone and sequence libraries of viral small RNAs from infected insect samples (mosquito, drosophilidae, insect-derived cell lines).

Domains: Molecular biology; Virology

Valérie Gausson, Maria-Carla P Saleh. Viral Small RNA Cloning and Sequencing. Antiviral RNAi: Concepts, Methods, and Applications, 721, Springer, pp.107-122, 2011, Methods in Molecular Biology, 978-1-61779-037-9. ⟨10.1007/978-1-61779-037-9_6⟩. ⟨pasteur-02089924⟩