Skip to Main content Skip to Navigation
New interface
Journal articles

A Blood RNA Signature Detecting Severe Disease in Young Dengue Patients at Hospital Arrival

Abstract : and 10 GSK vaccines R&D, Singapore Background. Early detection of severe dengue can improve patient care and survival. To date, no reliable single-gene biomarker exists. We hypothesized that robust multigene signatures exist. Methods. We performed a prospective study on Cambodian dengue patients aged 4 to 22 years. Peripheral blood mononuclear cells (PBMCs) were obtained at hospital admission. We analyzed 42 transcriptomic profiles of patients with secondary dengue infected with dengue serotype 1. Our novel signature discovery approach controls the number of included genes and captures non-linear relationships between transcript concentrations and severity. We evaluated the signature on secondary cases infected with different serotypes using 2 datasets: 22 PBMC samples from additional patients in our cohort and 32 whole blood samples from an independent cohort. Results. We identified an 18-gene signature for detecting severe dengue in patients with secondary infection upon hospital admission with a sensitivity of 0.93 (95% confidence interval [CI], .82-.98), specificity of 0.67 (95% CI, .53-.80), and area under the receiver operating characteristic curve (AUC) of 0.86 (95% CI, .75-.97). At validation, the signature had empirical AUCs of 0.85 (95% CI, .69-1.00) and 0.83 (95% CI, .68-.98) for the PBMCs and whole blood datasets, respectively. Conclusions. The signature could detect severe dengue in secondary-infected patients upon hospital admission. Its genes offer new insights into the pathogenesis of severe dengue.
Complete list of metadata

Cited literature [31 references]  Display  Hide  Download
Contributor : Anavaj Sakuntabhai Connect in order to contact the contributor
Submitted on : Wednesday, April 3, 2019 - 11:53:45 AM
Last modification on : Tuesday, October 25, 2022 - 4:23:27 PM


Publication funded by an institution


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License



Iryna Nikolayeva, Pierre Bost, Isabelle Casademont, Veasna Duong, Fanny Koeth, et al.. A Blood RNA Signature Detecting Severe Disease in Young Dengue Patients at Hospital Arrival. Journal of Infectious Diseases, 2018, 217 (11), pp.1690-1698. ⟨10.1093/infdis/jiy086⟩. ⟨pasteur-02088893⟩



Record views


Files downloads