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Journal articles
A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus
Abstract : BACKGROUND:
Chikungunya virus (CHIKV) is a mosquito-borne virus currently transmitted in about 60 countries. CHIKV causes acute flu-like symptoms and in many cases prolonged musculoskeletal and joint pain. Detection of the infection is mostly done using RT-RCR or ELISA, which are not suitable for point-of-care diagnosis.
METHODOLOGY/PRINCIPAL FINDINGS:
In this study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of the CHIKV was developed. The assay sensitivity, specificity, and cross-reactivity were tested. CHIKV RT-RPA assay detected down to 80 genome copies/reaction in a maximum of 15 minutes. It successfully identified 18 isolates representing the three CHIKV genotypes. No cross-reactivity was detected to other alphaviruses and arboviruses except O'nyong'nyong virus, which could be differentiated by a modified RPA primer pair. Seventy-eight samples were screened both by RT-RPA and real-time RT-PCR. The diagnostic sensitivity and specificity of the CHIKV RT-RPA assay were determined at 100%.
CONCLUSIONS/SIGNIFICANCE:
The developed RT-RPA assay represents a promising method for the molecular detection of CHIKV at point of need.
Cited literature [45 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-02083456
Contributor : Fanny Sebire <>
Submitted on : Friday, March 29, 2019 - 9:35:45 AM
Last modification on : Monday, December 14, 2020 - 3:46:14 PM
Long-term archiving on: : Sunday, June 30, 2019 - 12:58:37 PM
Contributor : Fanny Sebire <>
Submitted on : Friday, March 29, 2019 - 9:35:45 AM
Last modification on : Monday, December 14, 2020 - 3:46:14 PM
Long-term archiving on: : Sunday, June 30, 2019 - 12:58:37 PM
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journal.pntd.0004953.PDF
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Distributed under a Creative Commons Attribution 4.0 International License