1Imagerie et Modélisation - Imaging and Modeling (Département Biologie computationnelle,
Département de Biologie cellulaire et Infection,
25-28, rue du docteur Roux, 75724 Paris Cedex 15 - France)
Abstract : Single molecule localization microscopy can generate 3D super-resolution images without scanning by leveraging the axial variations of normal or engineered point spread functions (PSF). Successful implementation of these approaches for extended axial ranges remains, however, challenging. We present Zernike Optimized Localization Approach in 3D (ZOLA-3D), an easy-to-use computational and optical solution that achieves optimal resolution over a tunable axial range. We use ZOLA-3D to demonstrate 3D super-resolution imaging of mitochondria, nuclear pores and microtubules in entire nuclei or cells up to ~5 μm deep.
https://hal-pasteur.archives-ouvertes.fr/pasteur-02074371
Contributor : Marie Lemesle <>
Submitted on : Wednesday, March 20, 2019 - 3:50:59 PM Last modification on : Tuesday, December 8, 2020 - 9:37:38 AM Long-term archiving on: : Friday, June 21, 2019 - 9:17:42 PM