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Journal articles
Transport and Catabolism of Carbohydrates by Neisseria meningitidis.
Abstract : We identified the genes encoding the proteins for the transport of glucose and maltose in Neisseria meningitidis strain 2C4-3. A mutant deleted for NMV_1892(glcP) no longer grew on glucose and deletion of NMV_0424(malY) prevented the utilization of maltose. We also purified and characterized glucokinase and α-phosphoglucomutase, which catalyze early catabolic steps of the two carbohydrates. N. meningitidis catabolizes the two carbohydrates either via the Entner-Doudoroff (ED) pathway or the pentose phosphate pathway, thereby forming glyceraldehyde-3-P and either pyruvate or fructose-6-P, respectively. We purified and characterized several key enzymes of the two pathways. The genes required for the transformation of glucose into gluconate-6-P and its further catabolism via the ED pathway are organized in two adjacent operons. N. meningitidis also contains genes encoding proteins which exhibit similarity to the gluconate transporter (NMV_2230) and gluconate kinase (NMV_2231) of Enterobacteriaceae and Firmicutes. However, gluconate might not be the real substrate of NMV_2230 because N. meningitidis was not able to grow on gluconate as the sole carbon source. Surprisingly, deletion of NMV_2230 stimulated growth in minimal medium in the presence and absence of glucose and drastically slowed the clearance of N. meningitidis cells from transgenic mice after intraperitoneal challenge.
https://hal-pasteur.archives-ouvertes.fr/pasteur-02058783
Contributor : Marie Martin Connect in order to contact the contributor
Submitted on : Wednesday, March 6, 2019 - 10:42:48 AM
Last modification on : Tuesday, October 19, 2021 - 10:28:13 PM
Contributor : Marie Martin Connect in order to contact the contributor
Submitted on : Wednesday, March 6, 2019 - 10:42:48 AM
Last modification on : Tuesday, October 19, 2021 - 10:28:13 PM
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