Predicting susceptibility to tuberculosis based on gene expression profiling in dendritic cells - Institut Pasteur Access content directly
Journal Articles Scientific Reports Year : 2017

Predicting susceptibility to tuberculosis based on gene expression profiling in dendritic cells

Yoav Gilad
  • Function : Correspondent author
  • PersonId : 1043524

Connectez-vous pour contacter l'auteur

Abstract

Tuberculosis (TB) is a deadly infectious disease, which kills millions of people every year. The causative pathogen, Mycobacterium tuberculosis (MTB), is estimated to have infected up to a third of the world's population; however, only approximately 10% of infected healthy individuals progress to active TB. Despite evidence for heritability, it is not currently possible to predict who may develop TB. To explore approaches to classify susceptibility to TB, we infected with MTB dendritic cells (DCs) from putatively resistant individuals diagnosed with latent TB, and from susceptible individuals that had recovered from active TB. We measured gene expression levels in infected and non-infected cells and found hundreds of differentially expressed genes between susceptible and resistant individuals in the non-infected cells. We further found that genetic polymorphisms nearby the differentially expressed genes between susceptible and resistant individuals are more likely to be associated with TB susceptibility in published GWAS data. Lastly, we trained a classifier based on the gene expression levels in the non-infected cells, and demonstrated reasonable performance on our data and an independent data set. Overall, our promising results from this small study suggest that training a classifier on a larger cohort may enable us to accurately predict TB susceptibility.
Fichier principal
Vignette du fichier
41598_2017_Article_5878.pdf (3.2 Mo) Télécharger le fichier
Origin : Publication funded by an institution
Loading...

Dates and versions

pasteur-02046440 , version 1 (22-02-2019)

Licence

Attribution

Identifiers

Cite

John D Blischak, Ludovic Tailleux, Marsha Myrthil, Cécile Charlois, Emmanuel Bergot, et al.. Predicting susceptibility to tuberculosis based on gene expression profiling in dendritic cells. Scientific Reports, 2017, 7 (1), pp.5702. ⟨10.1038/s41598-017-05878-w⟩. ⟨pasteur-02046440⟩
39 View
96 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More