Methods for Studying Biofilm Dispersal in Pseudomonas aeruginosa

Nicolas Barraud (1) , Joana A Moscoso (2) , Jean-Marc Ghigo (3) , Alain Filloux (2)

1 UNSW - University of New South Wales [Sydney]
2 Imperial College London
3 Génétique des Biofilms

Biofilm dispersal is the last and least understood stage of the biofilm life cycle. Several recent studies have characterized dispersal events in response to various cues and signals. Here we describe a range of methods useful for the investigation of dispersal in the biofilm model organism and opportunistic pathogen Pseudomonas aeruginosa.

Keywords: Biofilm; Dispersal; Environmental cues; Nitric oxide; Batch culture; Crystal violet staining; Continuous culture; Microfermenters

Domains: Life Sciences [q-bio]; Microbiology and Parasitology

Nicolas Barraud, Joana A Moscoso, Jean-Marc Ghigo, Alain Filloux. Methods for Studying Biofilm Dispersal in Pseudomonas aeruginosa. Methods in Molecular Biology, 1149, Humana Press, pp.643-651, 2014, 978-1-4939-0472-3. ⟨10.1007/978-1-4939-0473-0_49⟩. ⟨pasteur-02017392⟩