, Midlog phase cultures were resuspended to 2 x 10 6 cells/ml and serially diluted tenfold. Dilutions were spotted on YES agar plates or YES agar containing the indicated amounts of hydroxyurea (HU) (Sigma), camptothecin (CPT) (Sigma), or methyl methanesulfonate (MMS)
, Lysates were pre-cleared with protein A agarose beads, followed by 2-hour incubation with antibodies against HA tag (Sigma, E6779) at 4 0 C. After washing, eluted proteins and input extracts were analyzed by Western blotting using antibodies against TAP, HB buffer 31, 2026.
, Additional references for methods
Molecular genetic analysis of fission yeast Schizosaccharomyces pombe, Methods Enzymol, vol.194, pp.795-823, 1991. ,
Assaying the Polyadenylation State of mRNAs, Methods, vol.17, pp.38-45 ,
CENP-B preserves genome integrity at replication forks paused by retrotransposon LTR, Nature, vol.469, pp.112-115, 2011. ,
URL : https://hal.archives-ouvertes.fr/pasteur-02013953
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, Genome Biol, vol.9, 2008.