DNA Synthesis by Primer Exchange Reaction Cascades

Marcel Hollenstein (1)

1 Chimie bioorganique des acides nucléiques - Bioorganic chemistry of nucleic acids

Swap and extend: The autonomous synthesis of single-stranded DNA molecules of arbitrary size and sequence composition can easily be achieved by primer exchange reaction (PER) cascades, in which the sequential polymerase-mediated extension of DNA primers is guided by catalytic hairpins. This highlight illustrates the potential of this method for applications in DNA nanotechnology.

Keywords: DNA; DNA synthesis; RNA; nanostructures; strand displacement

Domains: Chemical Sciences; Organic chemistry

Marcel Hollenstein. DNA Synthesis by Primer Exchange Reaction Cascades. ChemBioChem, 2018, 19 (5), pp.422-424. ⟨10.1002/cbic.201700639⟩. ⟨pasteur-02012260⟩