Skip to Main content Skip to Navigation
New interface
Journal articles

Incorporation of a minimal nucleotide into DNA

Abstract : Abasic sites are amongst the most frequent DNA lesions and result from spontaneous hydrolysis of the glycosidic bond or from the removal of damaged nucleobases. These depurination events can also occur on free deoxyribonucleoside triphosphates present in cells and lead to the formation of an abasic site triphosphate of which very little is known. Herein, we report the synthesis and biochemical characterization of the minimal triphosphate dФTP. Unexpectedly, dФTP is tolerated by various DNA polymerases and the incorporation efficiency obeys the A-rule. Single incorporation of dФMP units were also observed opposite abasic sites and the addition of prosthetic molecules mimicking base-pairs do not seem to favor the process.
Document type :
Journal articles
Complete list of metadata
Contributor : Marcel Hollenstein Connect in order to contact the contributor
Submitted on : Friday, February 8, 2019 - 3:34:05 PM
Last modification on : Friday, October 7, 2022 - 3:57:01 AM



Pascal Röthlisberger, Fabienne Levi-Acobas, Ivo Sarac, Rémy Ricoux, Jean-Pierre Mahy, et al.. Incorporation of a minimal nucleotide into DNA. Tetrahedron Letters, 2018, 59 (48), pp.4241-4244. ⟨10.1016/j.tetlet.2018.10.036⟩. ⟨pasteur-02012227⟩



Record views