Tetrahedral DNAzymes for enhanced intracellular gene-silencing activity

Hien Bao Dieu Thai (1, 2) , Fabienne Levi-Acobas (3) , Soo-Young Yum (4) , Goo Jang (4) , Marcel Hollenstein (3) , Dae-Ro Ahn (1, 2)

1 KISTI - Korea Institute of Science and Technology Information
2 UST - University of Science and Technology [Daejeon]
3 Chimie bioorganique des acides nucléiques - Bioorganic chemistry of nucleic acids
4 SNU - Seoul National University [Seoul]

We prepared tetrahedral DNAzymes (TDzs) to overcome potential limitations such as insufficient serum stability and poor cellular uptake of single-stranded DNAzymes (ssDzs). TDzs showed enhanced serum stability and higher cellular uptake efficiency compared to those of ssDzs, providing significantly improved intracellular gene-silencing activity to down-regulate the target mRNA level.

Domains: Chemical Sciences; Chemical Sciences; Organic chemistry

Hien Bao Dieu Thai, Fabienne Levi-Acobas, Soo-Young Yum, Goo Jang, Marcel Hollenstein, et al.. Tetrahedral DNAzymes for enhanced intracellular gene-silencing activity. Chemical Communications, 2018, 54 (68), pp.9410-9413. ⟨10.1039/c8cc05721d⟩. ⟨pasteur-02012225⟩