, Generating Ncr1-cre TG + ?c flox/y mice Set-up crosses of male Ncr1-cre TG + with female ?c flox/wt mice. This represents the optimal breeding scheme

, Place the lungs in a 24-well plate containing enough of a 30 µg/mL DLuciferin solution per well to cover the tissue and incubate them for 10 minutes at RT. When imaging more than one lung at the same time avoid placing the lungs in neighboring wells

, Image the plate without removing the solution

, Acknowledgements This work received funding to from the Institut Pasteur, INSERM, LNCC (Equipe Labellisée Ligue Contre le Cancer) and ANR

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