Protein-protein interactions leading to recruitment of the host DNA sliding clamp by the hyperthermophilic Sulfolobus islandicus rod-shaped virus 2

Andrew F. Gardner (1) , Stephen D. Bell (2) , Malcolm F. White (3) , David Prangishvili (4) , Mart Krupovic (4)

1 New England Biolabs
2 Indiana University [Bloomington]
3 University of St Andrews [Scotland]
4 BMGE - Biologie Moléculaire du Gène chez les Extrêmophiles

Viruses infecting hyperthermophilic archaea typically do not encode DNA polymerases, raising questions regarding their genome replication. Here, using a yeast two-hybrid approach, we have assessed interactions between proteins of Sulfolobus islandicus rod-shaped virus 2 (SIRV2) and the host-encoded proliferating cell nuclear antigen (PCNA), a key DNA replication protein in archaea. Five SIRV2 proteins were found to interact with PCNA, providing insights into the recruitment of host replisome for viral DNA replication.

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Microbiology and Parasitology; Life Sciences [q-bio]; Microbiology and Parasitology; Virology

Andrew F. Gardner, Stephen D. Bell, Malcolm F. White, David Prangishvili, Mart Krupovic. Protein-protein interactions leading to recruitment of the host DNA sliding clamp by the hyperthermophilic Sulfolobus islandicus rod-shaped virus 2. Journal of Virology, 2014, 88 (12), pp.7105--7108. ⟨10.1128/JVI.00636-14⟩. ⟨pasteur-01977399⟩