2Génétique fonctionnelle de la Souris (Département de Biologie du Développement et Cellules Souches - 25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
3Imagerie et Modélisation - Imaging and Modeling (Département Biologie computationnelle,
Département de Biologie cellulaire et Infection,
25-28, rue du docteur Roux, 75724 Paris Cedex 15 - France)
Abstract : Pluripotent mouse embryonic stem cells maintain their identity throughout virtually infinite cell divisions. This phenomenon, referred to as self-renewal, depends on a network of sequence-specific transcription factors (TFs) and requires daughter cells to accurately reproduce the gene expression pattern of the mother. However, dramatic chromosomal changes take place in mitosis, generally leading to the eviction of TFs from chromatin. Here, we report that Esrrb, a major pluripotency TF, remains bound to key regulatory regions during mitosis. We show that mitotic Esrrb binding is highly dynamic, driven by specific recognition of its DNA-binding motif and is associated with early transcriptional activation of target genes after completion of mitosis. These results indicate that Esrrb may act as a mitotic bookmarking factor, opening another perspective to molecularly understand the role of sequence-specific TFs in the epigenetic control of self-renewal, pluripotency and genome reprogramming.
https://hal-pasteur.archives-ouvertes.fr/pasteur-01972743 Contributor : Corinne BillionConnect in order to contact the contributor Submitted on : Monday, January 7, 2019 - 8:12:07 PM Last modification on : Thursday, April 7, 2022 - 10:10:35 AM Long-term archiving on: : Monday, April 8, 2019 - 6:49:34 PM