Improved reference genome of Aedes aegypti informs arbovirus vector control

Benjamin J. Matthews (1, 2, 3) , Olga Dudchenko (4, 5) , Sara Kingan (6) , Sergey Koren (7) , Igor Antoshechkin (8) , Jacob E. Crawford (9) , William J Glassford (10) , Margaret Herre (1, 3) , Seth N. Redmond (11, 12) , Noah H Rose (13) , Gareth D. Weedall (14) , Yang Wu (15, 16) , Sanjit S Batra (4, 5) , Carlos A Brito-Sierra (17) , Steven D Buckingham (18) , Corey L Campbell (19) , Saki Chan (20) , Eric Cox (21) , Benjamin R Evans (22) , Thanyalak Fansiri (23) , Igor Filipović (24) , Albin Fontaine (25, 26, 27) , Andrea Gloria-Soria (22) , Richard Hall (6) , Vinita S. Joardar (21) , Andrew K. Jones (28) , Raissa G G Kay (29) , Vamsi K. Kodali (21) , Joyce Lee (20) , Gareth J. Lycett (30) , Sarah N. Mitchell (9) , Jill Muehling (6) , Mickael R. Murphy (21) , Arina D Omer (4, 5) , Frederick A Partridge (18) , Paul Peluso (6) , Aviva Presser Aiden (4, 5) , Vidya Ramasamy (28) , Gordana Rašić (24) , Sourav Roy (29) , Karla Saavedra-Rodriguez (19) , Shruti Sharan (17) , Atashi Sharma (15) , Melissa Laird Smith (6) , Joe Turner (31) , Allison M. Weakley (9) , Zhilei Zhao (13) , Omar S Akbari (32) , William C Black (19) , Han Cao (20) , Alistair C. Darby (31) , Catherine A Hill (17) , J Spencer Johnston (33) , Terence Murphy (21) , Alex Raikhel (29) , David B. Sattelle (18) , Igor V. Sharakhov (15, 34) , Bradley J. White (9) , Li Zhao (1) , Erez Lieberman Aiden (4, 5, 11) , Richard S Mann (10) , Louis Lambrechts (25) , Jeffrey R Powell (22) , Maria Sharakhova (15, 34) , Zhijian Tu (15) , Hugh M Robertson (35) , Carolyn S Mcbride (13) , Alex R Hastie (20) , Jonas Korlach (6) , Daniel E Neafsey (11, 12) , Adam Phillippy (7) , Leslie B Vosshall (1, 3, 36)

1 Rockefeller University [New York]
2 HHMI - Howard Hughes Medical Institute [New York]
3 Kavli Neural Systems Institute
4 BCM - Baylor College of Medicine
5 Rice University [Houston]
6 Pacific Biosciences [Menlo Park]
7 NHGRI - National Human Genome Research Institute
8 CALTECH - California Institute of Technology
9 Verily Life Sciences Inc
10 Columbia University [New York]
11 BROAD INSTITUTE - Broad Institute of MIT and Harvard
12 Harvard T.H. Chan School of Public Health
13 Princeton University
14 LJMU - Liverpool John Moores University
15 Virginia Tech [Blacksburg]
16 Southern Medical University [Guangzhou]
17 Purdue University [West Lafayette]
18 UCL - University College of London [London]
19 CSU - Colorado State University [Fort Collins]
20 Bionano Genomics Inc
21 NCBI - National Center for Biotechnology Information
22 Yale University [New Haven]
23 AFRIMS - Armed Forces Research Institute of Medical Sciences [Bangkok]
24 QIMR Berghofer Medical Research Institute
25 IVI - Interactions Virus-Insectes - Insect-Virus Interactions
26 IRBA - Institut de Recherche Biomédicale des Armées [Antenne Marseille]
27 VITROME - Vecteurs - Infections tropicales et méditerranéennes
28 Oxford Brookes University
29 UC Riverside - University of California [Riverside]
30 LSTM - Liverpool School of Tropical Medicine
31 University of Liverpool
32 UC San Diego - University of California [San Diego]
33 Texas A&M University [College Station]
34 Tomsk State University [Tomsk]
35 UIC - University of Illinois [Chicago]
36 Howard Hughes Medical Institute [New York]

Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. Here we combine diverse technologies to produce the markedly improved, fully re-annotated AaegL5 genome assembly, and demonstrate how it accelerates mosquito science. We anchored physical and cytogenetic maps, doubled the number of known chemosensory ionotropic receptors that guide mosquitoes to human hosts and egg-laying sites, provided further insight into the size and composition of the sex-determining M locus, and revealed copy-number variation among glutathione S-transferase genes that are important for insecticide resistance. Using high-resolution quantitative trait locus and population genomic analyses, we mapped new candidates for dengue vector competence and insecticide resistance. AaegL5 will catalyse new biological insights and intervention strategies to fight this deadly disease vector.

Domaines: Sciences du Vivant [q-bio]; Virologie; Génomique, Transcriptomique et Protéomique [q-bio.GN]; Génétique; Interactions entre organismes

Benjamin J. Matthews, Olga Dudchenko, Sara Kingan, Sergey Koren, Igor Antoshechkin, et al.. Improved reference genome of Aedes aegypti informs arbovirus vector control. Nature, 2018, 563 (7732), pp.501-507. ⟨10.1038/s41586-018-0692-z⟩. ⟨pasteur-01969223⟩