Journal articles

Improved reference genome of Aedes aegypti informs arbovirus vector control

Benjamin J. Matthews ^{1, 2, 3} Olga Dudchenko ^{4, 5} Sara Kingan ⁶ Sergey Koren ⁷ Igor Antoshechkin ⁸ Jacob E. Crawford ⁹ William J Glassford ¹⁰ Margaret Herre ^{1, 3} Seth N. Redmond ^{11, 12} Noah H Rose ¹³ Gareth D. Weedall ¹⁴ yang Wu ^{15, 16} Sanjit S Batra ^{4, 5} Carlos A Brito-Sierra ¹⁷ Steven D Buckingham ¹⁸ Corey L Campbell ¹⁹ Saki Chan ²⁰ Eric Cox ²¹ Benjamin R Evans ²² Thanyalak Fansiri ²³ Igor Filipović ²⁴ Albin Fontaine ^{25, 26, 27} Andrea Gloria-Soria ²² Richard Hall ⁶ Vinita S. Joardar ²¹ Andrew K. Jones ²⁸ Raissa G G Kay ²⁹ Vamsi K. Kodali ²¹ Joyce Lee ²⁰ Gareth J. Lycett ³⁰ Sarah N. Mitchell ⁹ Jill Muehling ⁶ Mickael R. Murphy ²¹ Arina D Omer ^{4, 5} Frederick A Partridge ¹⁸ Paul Peluso ⁶ Aviva Presser Aiden ^{4, 5} Vidya Ramasamy ²⁸ Gordana Rašić ²⁴ Sourav Roy ²⁹ Karla Saavedra-Rodriguez ¹⁹ Shruti Sharan ¹⁷ Atashi Sharma ¹⁵ Melissa Laird Smith ⁶ Joe Turner ³¹ Allison M. Weakley ⁹ Zhilei Zhao ¹³ Omar S Akbari ³² William C Black ¹⁹ Han Cao ²⁰ Alistair C. Darby ³¹ Catherine A Hill ¹⁷ J Spencer Johnston ³³ Terence Murphy ²¹ Alex Raikhel ²⁹ David B. Sattelle ¹⁸ Igor V. Sharakhov ^{15, 34} Bradley J. White ⁹ Li Zhao ¹ Erez Lieberman Aiden ^{4, 5, 11} Richard S Mann ¹⁰ Louis Lambrechts ²⁵ Jeffrey R Powell ²² Maria Sharakhova ^{15, 34} Zhijian Tu ¹⁵ Hugh M Robertson ³⁵ Carolyn S Mcbride ¹³ Alex R Hastie ²⁰ Jonas Korlach ⁶ Daniel E Neafsey ^{11, 12} Adam Phillippy ⁷ Leslie B Vosshall ^{1, 3, 36}

1 Rockefeller University [New York]

2 HHMI - Howard Hughes Medical Institute [New York]

3 Kavli Neural Systems Institute

4 BCM - Baylor College of Medicine

5 Rice University [Houston]

6 Pacific Biosciences [Menlo Park]

7 NHGRI - National Human Genome Research Institute

8 CALTECH - California Institute of Technology

9 Verily Life Sciences Inc

10 Columbia University [New York]

11 BROAD INSTITUTE - Broad Institute of MIT and Harvard

12 Harvard T.H. Chan School of Public Health

13 Princeton University

14 LJMU - Liverpool John Moores University

15 Virginia Tech [Blacksburg]

16 Southern Medical University [Guangzhou]

17 Purdue University [West Lafayette]

18 UCL - University College of London [London]

19 CSU - Colorado State University [Fort Collins]

20 Bionano Genomics Inc

21 NCBI - National Center for Biotechnology Information

22 Yale University [New Haven]

23 AFRIMS - Armed Forces Research Institute of Medical Sciences [Bangkok]

24 QIMR Berghofer Medical Research Institute

25 IVI - Interactions Virus-Insectes - Insect-Virus Interactions

26 IRBA - Institut de Recherche Biomédicale des Armées [Antenne Marseille]

27 VITROME - Vecteurs - Infections tropicales et méditerranéennes

28 Oxford Brookes University

29 UCR - University of California [Riverside]

30 LSTM - Liverpool School of Tropical Medicine

31 University of Liverpool

32 UC San Diego - University of California [San Diego]

33 Texas A&M University [College Station]

34 Tomsk State University [Tomsk]

35 UIC - University of Illinois [Chicago]

36 Howard Hughes Medical Institute [New York]

Abstract : Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. Here we combine diverse technologies to produce the markedly improved, fully re-annotated AaegL5 genome assembly, and demonstrate how it accelerates mosquito science. We anchored physical and cytogenetic maps, doubled the number of known chemosensory ionotropic receptors that guide mosquitoes to human hosts and egg-laying sites, provided further insight into the size and composition of the sex-determining M locus, and revealed copy-number variation among glutathione S-transferase genes that are important for insecticide resistance. Using high-resolution quantitative trait locus and population genomic analyses, we mapped new candidates for dengue vector competence and insecticide resistance. AaegL5 will catalyse new biological insights and intervention strategies to fight this deadly disease vector.

Document type :

Journal articles

Domain :

Life Sciences [q-bio]

Life Sciences [q-bio] / Microbiology and Parasitology / Virology

Life Sciences [q-bio] / Biochemistry, Molecular Biology / Genomics [q-bio.GN]

Life Sciences [q-bio] / Genetics

Life Sciences [q-bio] / Ecology, environment / Symbiosis

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Submitted on : Thursday, January 3, 2019 - 7:07:25 PM
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