Effect of SIVmac infection on plasmacytoid and CD1c + myeloid dendritic cells in cynomolgus macaques

Benoît Malleret (1) , Ingrid Karlsson (1) , Benjamin Manéglier (2, 3) , Patricia Brochard (1) , Benoît Delache (1) , Thibault Andrieu (1) , Michaela Muller-Trutwin (4) , Tim Beaumont (5) , Joseph M Mccune (5) , Jacques Banchereau (6) , Roger Le Grand (1) , Bruno Vaslin (1)

1 IMETI - Institut des Maladies Emergentes et des Thérapies Innovantes
2 CHV - Centre Hospitalier de Versailles André Mignot
3 UVSQ - Université de Versailles Saint-Quentin-en-Yvelines
4 Régulation des Infections Rétrovirales
5 UC San Francisco - University of California [San Francisco]
6 BIIR - Baylor Institute for Immunology Research

Dendritic cells (DCs) are known to be essential for the induction and regulation of immune responses. Non-human primates are essential in biomedical research and contribute to our understanding of the involvement of DCs in human infectious diseases. However, no direct single-platform method for quantifying DC precursors has yet been optimized in macaques to give accurate absolute blood counts of these rare-event cell populations in the blood. We adapted a rapid whole-blood assay for the absolute quantification of DCs in cynomolgus macaques by four-colour flow cytometry, using a single-platform assay compatible with human blood. Cynomolgus macaque plasmacytoid DCs (pDCs) and CD1c(+) myeloid DCs (CD1c(+) mDCs) were quantified in the blood of 34 healthy macaques and the results obtained were compared with those for blood samples from 11 healthy humans. In addition, circulating absolute numbers of pDCs were quantified in cynomolgus macaques chronically infected with SIVmac. During infection, pDC counts decreased whereas circulating CD1c(+) mDC counts increased. Information regarding absolute pDC and mDC counts in non-human primates may improve our understanding of the role of these cells in SIV/HIV infection and in other infectious diseases.

Keywords: Cynomolgus macaque; Dendritic cells; Absolute counts; SIV; AIDS

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Immunology; Life Sciences [q-bio]; Human health and pathology; Infectious diseases; Life Sciences [q-bio]; Microbiology and Parasitology; Virology; Life Sciences [q-bio]; Cellular Biology

Benoît Malleret, Ingrid Karlsson, Benjamin Manéglier, Patricia Brochard, Benoît Delache, et al.. Effect of SIVmac infection on plasmacytoid and CD1c + myeloid dendritic cells in cynomolgus macaques. Immunology, 2008, 124 (2), pp.223-233. ⟨10.1111/j.1365-2567.2007.02758.x⟩. ⟨pasteur-01962624⟩