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Journal articles
Bidirectional intraflagellar transport is restricted to two sets of microtubule doublets in the trypanosome flagellum
Abstract : Intraflagellar transport (IFT) is the rapid bidirectional movement of large protein complexes driven by kinesin and dynein motors along microtubule doublets of cilia and flagella. In this study, we used a combination of high-resolution electron and light microscopy to investigate how and where these IFT trains move within the flagellum of the protist Trypanosoma brucei Focused ion beam scanning electron microscopy (FIB-SEM) analysis of trypanosomes showed that trains are found almost exclusively along two sets of doublets (3-4 and 7-8) and distribute in two categories according to their length. High-resolution live imaging of cells expressing mNeonGreen::IFT81 or GFP::IFT52 revealed for the first time IFT trafficking on two parallel lines within the flagellum. Anterograde and retrograde IFT occurs on each of these lines. At the distal end, a large individual anterograde IFT train is converted in several smaller retrograde trains in the space of 3-4 s while remaining on the same side of the axoneme.
Cited literature [88 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-01931458
Contributor : Philippe Bastin Connect in order to contact the contributor
Submitted on : Thursday, November 22, 2018 - 6:14:40 PM
Last modification on : Tuesday, January 4, 2022 - 3:54:21 AM
Long-term archiving on: : Saturday, February 23, 2019 - 3:50:34 PM
Contributor : Philippe Bastin Connect in order to contact the contributor
Submitted on : Thursday, November 22, 2018 - 6:14:40 PM
Last modification on : Tuesday, January 4, 2022 - 3:54:21 AM
Long-term archiving on: : Saturday, February 23, 2019 - 3:50:34 PM
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