Skip to Main content Skip to Navigation
New interface
Journal articles

Macropinosomes are Key Players in Early Shigella Invasion and Vacuolar Escape in Epithelial Cells

Abstract : Intracellular pathogens include all viruses, many bacteria and parasites capable of invading and surviving within host cells. Key to survival is the subversion of host cell pathways by the pathogen for the purpose of propagation and evading the immune system. The intracellular bacterium Shigella flexneri, the causative agent of bacillary dysentery, invades host cells in a vacuole that is subsequently ruptured to allow growth of the pathogen within the host cytoplasm. S. flexneri invasion has been classically described as a macropinocytosis-like process, however the underlying details and the role of macropinosomes in the intracellular bacterial lifestyle have remained elusive. We applied dynamic imaging and advanced large volume correlative light electron microscopy (CLEM) to study the highly transient events of S. flexneri's early invasion into host epithelial cells and elucidate some of its fundamental features. First, we demonstrate a clear distinction between two compartments formed during the first step of invasion: the bacterial containing vacuole and surrounding macropinosomes, often considered identical. Next, we report a functional link between macropinosomes and the process of vacuolar rupture, demonstrating that rupture timing is dependent on the availability of macropinosomes as well as the activity of the small GTPase Rab11 recruited directly to macropinosomes. We go on to reveal that the bacterial containing vacuole and macropinosomes come into direct contact at the onset of vacuolar rupture. Finally, we demonstrate that S. flexneri does not subvert pre-existing host endocytic vesicles during the invasion steps leading to vacuolar rupture, and propose that macropinosomes are the major compartment involved in these events. These results provide the basis for a new model of the early steps of S. flexneri epithelial cell invasion, establishing a different view of the enigmatic process of cytoplasmic access by invasive bacterial pathogens.
Complete list of metadata

Cited literature [51 references]  Display  Hide  Download
Contributor : Laurence Langlais Connect in order to contact the contributor
Submitted on : Friday, October 19, 2018 - 2:52:35 PM
Last modification on : Thursday, April 7, 2022 - 10:10:35 AM
Long-term archiving on: : Sunday, January 20, 2019 - 2:53:38 PM


Distributed under a Creative Commons Attribution 4.0 International License




Allon Weiner, Nora Mellouk, Noelia López-Montero, Yuen-Yan Chang, Célia Souque, et al.. Macropinosomes are Key Players in Early Shigella Invasion and Vacuolar Escape in Epithelial Cells. PLoS Pathogens, 2016, 12 (5), pp.e1005602. ⟨10.1371/journal.ppat.1005602⟩. ⟨pasteur-01899441⟩



Record views


Files downloads