First description of agonist and antagonist IP-10 in urine of patients with active TB

Linda Petrone (1) , Vincent Bondet (2) , Valentina Vanini (1) , Gilda Cuzzi (1) , Fabrizio Palmieri (1) , Ivana Palucci (3) , Giovanni Delogu (3) , Fabiola Ciccosanti (1) , Gian Maria Fimia (1, 4) , Thomas Blauenfeldt (5) , Morten Ruhwald (5) , Darragh Duffy (2) , Delia Goletti (1)

1 INMI - Istituto Nazionale di Malattie Infettive "Lazzaro Spallanzani"
2 Immunobiologie des Cellules dendritiques
3 Fondazione Policlinico Universitario Agostino Gemelli IRCCS
4 University of Salento [Lecce]
5 Statens Serum Institut [Copenhagen]

Objectives Biomarkers for tuberculosis (TB) diagnosis and clinical management are needed to defeat TB. In chronic hepatitis, patients not responding to interferon/ribavirin treatment had high levels of an antagonist form of IP-10. Recently, antagonist IP-10 has been showed to be involved also in TB pathogenesis. Here, we investigated IP-10 agonist/antagonist forms as potential inflammatory biomarkers to support TB diagnosis and monitoring. Methods Total IP-10 and its agonist/antagonist forms were measured by SIMOA digital ELISA in urine obtained from patients with active TB at baseline and after treatment. Healthy donors (HD) and patients with pneumonia were enrolled as controls. Results Patients with active TB had significantly higher levels of total and agonist IP-10 at baseline compared to HD; conversely, no differences were observed between IP-10 levels in active TB vs pneumonia. Moreover, in active TB a decline of total urine IP-10 was observed at therapy completion; agonist/antagonist forms reflected this decline although their differences were not statistically significant. Conclusions We showed for the first time that agonist/antagonist IP-10 forms are measurable in urine. IP-10 levels associate with TB and pneumonia disease, suggesting their association with acute inflammation. Further studies are needed to assess their role to monitor TB treatment efficacy.

Keywords: Biomarker; IP-10 antagonism; Treatment monitoring; Tuberculosis; Urine

Domains: Life Sciences [q-bio]; Immunology

Linda Petrone, Vincent Bondet, Valentina Vanini, Gilda Cuzzi, Fabrizio Palmieri, et al.. First description of agonist and antagonist IP-10 in urine of patients with active TB. International Journal of Infectious Diseases, 2018, ⟨10.1016/j.ijid.2018.09.001⟩. ⟨pasteur-01880632⟩