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Human thymopoiesis is influenced by a common genetic variant within the TCRA-TCRD locus

Emmanuel Clave 1, 2 Itauá Leston Araujo 1, 2 Cécile Alanio 3, 4 Etienne Patin 5, 6 Jacob Bergstedt 7 Alejandra Urrutia 3, 4, 8 Silvia Lopez-Lastra 9 Yan Li 9 Bruno Charbit 4 Cameron Ross Macpherson 4 Milena Hasan 4 Breno Luiz Melo-Lima 2, 1 Corinne Douay 2, 1 Noémie Saut 10, 11 Marine Germain 12 David-Alexandre Tregouet 12 Pierre-Emmanuel Morange 10, 11 Magnus Fontes 13, 14 Darragh Duffy 3, 4 James Di Santo 9 Lluis Quintana-Murci 5, 6 Matthew Albert 14, 3, 4, * Antoine Toubert 2, 1, 15, * Consortium The Milieu Interieur
* Corresponding author
1 IUH - Institut Universitaire d'Hématologie
2 A2T - Alloimmunité-Autoimmunité-Transplantation
3 Immunobiologie des Cellules dendritiques
4 CRT - Centre de Recherche Translationnelle
5 Génétique Evolutive Humaine - Human Evolutionary Genetics
6 C3BI - Centre de Bioinformatique, Biostatistique et Biologie Intégrative
7 Lund University [Lund]
8 Genentech, Inc.
9 Immunité Innée
10 Laboratoire d'hématologie biologique [Hôpital de la Timone - Hôpital Nord - APHM]
11 C2VN - Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research
12 ICAN - Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition
13 IGDA - International Group for Data Analysis
Institut Pasteur [Paris]
14 Genentech, Inc. [San Francisco]
15 Service d'Immunologie et d'Histocompatibilité
Abstract : The thymus is the primary lymphoid organ where naïve T cells are generated; however, with the exception of age, the parameters that govern its function in healthy humans remain unknown. We characterized the variability of thymic function among 1000 age- and sex-stratified healthy adults of the Milieu Intérieur cohort, using quantification of T cell receptor excision circles (TRECs) in peripheral blood T cells as a surrogate marker of thymopoiesis. Age and sex were the only nonheritable factors identified that affect thymic function. TREC amounts decreased with age and were higher in women compared to men. In addition, a genome-wide association study revealed a common variant (rs2204985) within the T cell receptor TCRA-TCRD locus, between the DD2 and DD3 gene segments, which associated with TREC amounts. Strikingly, transplantation of human hematopoietic stem cells with the rs2204985 GG genotype into immunodeficient mice led to thymopoiesis with higher TRECs, increased thymocyte counts, and a higher TCR repertoire diversity. Our population immunology approach revealed a genetic locus that influences thymopoiesis in healthy adults, with potentially broad implications in precision medicine.
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Journal articles
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01880378
Contributor : Marie-Christine Vougny <>
Submitted on : Monday, September 24, 2018 - 5:11:33 PM
Last modification on : Monday, December 14, 2020 - 5:22:03 PM

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PASTEUR | CNRS | INRA | ATP-IMMUN | UNIV-AMU | SORBONNE-UNIVERSITE | ICAN | SU-MEDECINE | UNIV-PARIS7 | USPC | INRAE | UNIV-PARIS | SU-TI

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Emmanuel Clave, Itauá Leston Araujo, Cécile Alanio, Etienne Patin, Jacob Bergstedt, et al.. Human thymopoiesis is influenced by a common genetic variant within the TCRA-TCRD locus. Science Translational Medicine, American Association for the Advancement of Science, 2018, 10 (457), pp.eaao2966. ⟨10.1126/scitranslmed.aao2966⟩. ⟨pasteur-01880378⟩

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