Skip to Main content Skip to Navigation
New interface
Journal articles

RNA interference is essential for cellular quiescence

Abstract : Quiescent cells play a predominant role in most organisms. Here we identify RNA interference (RNAi) as a major requirement for quiescence (G0 phase of the cell cycle) in Schizosaccharomyces pombe RNAi mutants lose viability at G0 entry and are unable to maintain long-term quiescence. We identified suppressors of G0 defects in cells lacking Dicer (dcr1Δ), which mapped to genes involved in chromosome segregation, RNA polymerase-associated factors, and heterochromatin formation. We propose a model in which RNAi promotes the release of RNA polymerase in cycling and quiescent cells: (i) RNA polymerase II release mediates heterochromatin formation at centromeres, allowing proper chromosome segregation during mitotic growth and G0 entry, and (ii) RNA polymerase I release prevents heterochromatin formation at ribosomal DNA during quiescence maintenance. Our model may account for the codependency of RNAi and histone H3 lysine 9 methylation throughout eukaryotic evolution.
Document type :
Journal articles
Complete list of metadata

Cited literature [24 references]  Display  Hide  Download
Contributor : Benoit Arcangioli Connect in order to contact the contributor
Submitted on : Wednesday, February 13, 2019 - 2:41:41 PM
Last modification on : Monday, May 9, 2022 - 11:58:08 AM


Distributed under a Creative Commons Attribution 4.0 International License




B. Roche, Benoît Arcangioli, R. Martienssen. RNA interference is essential for cellular quiescence. Science, 2016, 354 (6313), ⟨10.1126/science.aah5651⟩. ⟨pasteur-01868276⟩



Record views


Files downloads