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Journal articles
RNA interference is essential for cellular quiescence
Abstract : Quiescent cells play a predominant role in most organisms. Here we identify RNA interference (RNAi) as a major requirement for quiescence (G0 phase of the cell cycle) in Schizosaccharomyces pombe RNAi mutants lose viability at G0 entry and are unable to maintain long-term quiescence. We identified suppressors of G0 defects in cells lacking Dicer (dcr1Δ), which mapped to genes involved in chromosome segregation, RNA polymerase-associated factors, and heterochromatin formation. We propose a model in which RNAi promotes the release of RNA polymerase in cycling and quiescent cells: (i) RNA polymerase II release mediates heterochromatin formation at centromeres, allowing proper chromosome segregation during mitotic growth and G0 entry, and (ii) RNA polymerase I release prevents heterochromatin formation at ribosomal DNA during quiescence maintenance. Our model may account for the codependency of RNAi and histone H3 lysine 9 methylation throughout eukaryotic evolution.
Cited literature [24 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-01868276
Contributor : Benoit Arcangioli Connect in order to contact the contributor
Submitted on : Wednesday, February 13, 2019 - 2:41:41 PM
Last modification on : Thursday, December 2, 2021 - 11:22:01 AM
Contributor : Benoit Arcangioli Connect in order to contact the contributor
Submitted on : Wednesday, February 13, 2019 - 2:41:41 PM
Last modification on : Thursday, December 2, 2021 - 11:22:01 AM
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